中华皮肤科杂志 ›› 1993, Vol. 26 ›› Issue (6): 354-356.

• 实验研究 • 上一篇    下一篇

高亲和力IgE-he受体介导IgE合至郎格罕细胞

王秉鹤1, Rieger A Stingl G Ochiai K Kinet JP2   

  1. 1. 南京医学院第一附属医院皮肤科 210029;
    2. 奥地利维也纳大学医学院皮肤科;
    3. 美国国立卫生研究院(NIH)
  • 收稿日期:1992-10-19 修回日期:1993-03-23 出版日期:1993-12-15 发布日期:1993-12-15

FcεRI Mediates IgE Binding to Langerhans Cells

WANG Bing-He1, Armin Rieger2   

  • Received:1992-10-19 Revised:1993-03-23 Online:1993-12-15 Published:1993-12-15

摘要: 正常人表皮郎格罕细胞(LC)能特异性结合IgiJ,这种结合能被针对高亲和力IgE-Fc受体(FcERI)单克隆抗体(mAb)15-1完全阻断.然而,抗低亲和力IgE-Fc受体(FcsRII)mAb MHM、抗II型IgG-Fc受体(FcγRII)mAb IV3或乳精则不能阻断IgE结合至LC.这些观察结果提示IgE结合至LC是通过FcεRI,而不是FceRII,FcyRII或IgE结合蛋白(eBP)所介导.免疫标记研究进一步显示抗FcεRI mAb能特异性标记LC表面本研究首次证明表皮LC具有FcεRI.

关键词: Langerhans细胞, 免痊球蛋白E类, 高亲和力IgE-Ec受体

Abstract: Epidermal Laugerhans cells(LC) from normal human skin can specifically bind IgE.1gE-binding to LC could be entirely abrogated by pre-incubation of the akin tissue with the anti-FczRI mAb 15-1.However,the binding could neither be prevented by the anti-FcsRII mAh MHM6,anti-FcγRII mAb Ⅳ,3 nor lactase.These observations indicate that IgE.-binding to LC is mediated by FcεRI rather than by FcεRII,FcγRII or IgE-binding protein(εBP).Immunolabeling study showed that LC exhibited distinct surface labeling with anti-FcεRI mAb.The present study for the fist time demonstrates that epidermal LCs bear FcεRI.

Key words: Langerhans cells FcεRI, IgE