中华皮肤科杂志 ›› 1993, Vol. 26 ›› Issue (6): 340-342.

• 论著 • 上一篇    下一篇

聚合酶链反应及组织病理诊断尖锐湿疣的比较研究

孔繁荣1, 朱学骏1, 陈洪2, 翟文学2, 朱衡2, 朱立煌2   

  1. 1. 北京医科大学第一医院皮肤科 100034;
    2. 中国科学院遗传研究所
  • 收稿日期:1992-11-25 修回日期:1993-02-23 出版日期:1993-12-15 发布日期:1993-12-15

A Comparative Study on Diagnosis of Condyloma Accuminatum by Polymerase Chain Reaction and Histopathology

KONG Fan-Rong1, ZHU Xue-Jun1   

  1. The First Teaching Hospital of Beijing Medical University, Beijing 100034
  • Received:1992-11-25 Revised:1993-02-23 Online:1993-12-15 Published:1993-12-15

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摘要： 运用聚合酶链反应及组织病理诊断20例尖锐湿沈(CA),结果表明PCR诊断CA具有特异性强、灵敏度高及方法简便的特点,组织病理是诊断CA的一个重要手段,但有时会出现假阴性,作者对PCR的特异扩增片段进行了克隆和序列测定,其结果进一步肯定了本方法的可靠性.

关键词: 聚合酶链反应, 尖锐湿疣

Abstract: The authors reported the results of diagnosis of Condyloma accuminatung(CA) by poly-merase chain reaction(PCR) and histopa-thology.The results showed that diagnosis by PCR has high specificity,sensitivity and is easy to handle.Histopathology is one of the main diagnostic method of CA,however,there are false negative results.The authors further cloned and sequenced the specific DNA segmeet amplified by PCR.It confirmed the reliability of PCR technique.

Key words: Condylomata, acuminata, Polymerase chain reaction