中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (1): 22-25,62.

• 论著 • 上一篇    下一篇

白含珠菌体外粘附条件的探讨

郭宁如, 吴绍熙, 吕桂霞   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京210042
  • 收稿日期:1992-12-23 修回日期:1993-04-14 出版日期:1994-02-15 发布日期:1994-02-15
  • 基金资助:
    自然科学基金资助课题

In Vitro Study on the Adherence Con ditions of Candida Albicans

GUO Ning-Ru, WU Shao-Xi, LÜ Gui-Xia   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1992-12-23 Revised:1993-04-14 Online:1994-02-15 Published:1994-02-15

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摘要： 以正交设计法,采用了正交表L8(41×24)研究了念珠菌对上皮细胞体外吸附的诸条件,包括4因素及2～4水平,分别比较各因素在不同水平下的吸附率。结果提示,促进吸附的最佳组合因素是:念珠菌菌相(E=34.26),孵育12小时(D=33.70),念珠菌与细胞间比率为1:200(C=34.88)以及孵育温度在37℃时(B=33.86).以4种物质孵育后均可抑制菌对细胞的抑制率,其中尤以甘草甜素的作用为最强。由此也提示了器官表面(如口腔,肠道)常驻的真菌的数量对念珠菌病的发病极为重要,如能服用一些中药,如甘草甜素将是可能控制发生念珠菌病的一种措施。

关键词: 白念珠菌, 粘附

Abstract: In order to explore the in vitro conditions about the adherence of Candida to epithelium, an orthogonal design L8 (41×24) including 4 factors and 2-4 levels to compare the percentage of adherences in different levels of each factor was tested. The results showeds that the optimum combination factors enhancing adherence are as follows: phase of Candida (E=34.26); incubation period 12 h (D=33.70); ratio of Candida to cultured cells l:200 (C=34.88); incubation temperature 37 (B=33.86). All four material. tested could inhibit the adherence of C. albicans, among which glycirrhizine is the most poteds. It also indicated that the hequantity of fungi colonized on the surfaces of organs (mouth, intestine) is important to the pathogenesis of candidiasis, and the administration of some Chinese herbal medicines such as glycyrrizine probably could protect hosts from candidiasis.

Key words: Candida albicans, Abherence