中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (3): 152-154,190.

• 论著 • 上一篇    下一篇

新型隐球菌核型的脉冲电泳分析

刘维达1, 吴绍熙1, 柴建华2, 郭宁如1   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042;
    2. 复旦大学遗传所
  • 收稿日期:1993-06-30 修回日期:1993-10-06 出版日期:1994-06-15 发布日期:1994-06-15

Karyotype Analysis of Cryptococcus Neojormans by Pulsed-rield Gel Electrophoresls

LIU Wei-Da1, WU Shao-Xi1, CAI Jian-Hua2   

  1. Institute of Dermatology, Peking Union Medical College and Chinese Academy of Medical Sciences, Nanjing 210042
  • Received:1993-06-30 Revised:1993-10-06 Online:1994-06-15 Published:1994-06-15

PDF (PC)

340

赞

摘要： 用脉冲电泳技术分离了包括3个变种、5种血清型在内的20株新型隐球菌染色体DNA,发现其基因组由10～12条染色体组成,大小在0.4～2.1Mb之间,总长度约15Mb.特别是注意到电泳核型与血清型之间有相关性,并观察到菌株间的带型也存在不同程度的差异,提示电泳核型分析可作为变种鉴别和种内分型的新的基因型方法.

关键词: 新型隐球菌, 电泳,脉冲场凝胶

Abstract: Chromosomes from Cryptococcus neoformans were separated by pulsed-field gel electrophoresis.Twenty strains of C.neoformans including all five serotypes and three var.species were studied.The results showed the genomes of C.neoformans are composed of 10-12 chromosomes ranging in size from 0.4-2.1 Mb,and total DNA is about 15 Mb.A good correlation was observed between the electrophoretic karyotype and the serotype of C.neoformans.It was found that each strain had a unique,reproducible pattern of chromosome bands,so this strain polymorphism can be used as an epidemiologic marker of C.neoformans infection.

Key words: Cryptococcus neoformans, Electrophoresis,pulsed-field gel