中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (6): 339-341,398.

• 论著 • 上一篇    下一篇

常见病原念珠菌鉴定的数码分类方法

李安生, 吕桂霞, 郭宁如, 吴绍熙   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京, 210042
  • 收稿日期:1994-02-16 修回日期:1994-06-08 发布日期:1994-12-15
  • 基金资助:
    中国医学科学院、中国协和医科大学青年科学研究基金课题

Identification of Clinical Candida Isolates by a Coding Method

LI An-Sheng, LÜ, Gut-Xia, GUO Ning-Ru   

  1. Iustitute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1994-02-16 Revised:1994-06-08 Published:1994-12-15

摘要: 建立了一种用于常见病原性念珠菌菌种鉴定的多种碳源同化试验及结果分析的数码分类方法.结果,对14种碳源固相同化试验结果的编码分析可形成5位数码,进而形成18种数码可明确区分8种受试的病原念珠菌.结果表明,多种碳源同化试验及编码分析方法可以快速、明确地鉴定8种临床最常见的念珠菌,也为今后建立临床酵母菌快速鉴定系统打下了基础.

关键词: 念珠菌鉴定, 念珠菌属

Abstract: A coding method was established to identify medically important Candida species (C.albicans, C.glabrata, C.guilliermondii, C.kefyr, C.krusei, C.parapsilosis, C.stellatoidea and C.tropicalis).Firstly, one l.lundred and eleven clinical isolates were identified by the conventional methods into six Candida species lisTed above except for C.kefyr and C.stellatoidea.Then, the clinical isolates and 44 reference strains were examined with 14 carbonhydrate assimilation tests.The results of assimilation tests of each isolate were processed by a coding method into a 5-digital code.Finally, 8 Candida isolates to be tested could be expressed in 18 represelltative codes.The method established can be used for the rapid identification of clinical yeast isolates in clinical laboratory.

Key words: Candida, Candida identification