中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (4): 235-237.

• 论著 • 上一篇    下一篇

白念珠菌分泌型天冬氨酸蛋白酶基因在急性念珠菌性外阴阴道炎中的表达

冯静, 涂亚庭, 林能兴, 冯爱平   

  1. 华中科技大学同济医学院附属协和医院皮肤科 武汉 430022
  • 收稿日期:2004-04-06 出版日期:2005-04-15 发布日期:2005-04-15
  • 通讯作者: 涂亚庭,E-mail:tjhappy@126.com E-mail:tjhappy@126.com

Expression of Candida albicans Secreted Aspartyl Proteinase in Acute Vaginal Candidiasis

FENG Jing, TU Ya-ting, LING Neng-xing, FENG Ai-ping   

  1. Department of Dermatology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • Received:2004-04-06 Online:2005-04-15 Published:2005-04-15

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摘要： 目的 了解白念珠菌分泌型天冬氨酸蛋白酶(Sap)基因在人类阴道白念珠菌感染时的表达情况。方法 收集9例阴道念珠菌检测阴性及20例阴道念珠菌感染者的阴道分泌物,通过用特异引物系列进行RT-PCR反应来评价Sap1~Sap6在人体阴道环境中的表达情况。结果 Sap2和Sap5在阴道白念珠菌感染者中是最主要的表达基因;Sap3和Sap4在所有检查者中均被检测到;所有6种Sap基因在某些阴道念珠菌病患者中同时被检出。结论 Sap基因可能与阴道念珠菌病的发病机制有关。

关键词: 念珠菌病,外阴阴道, 念珠菌,白色, 天冬氨酸内肽酶类

Abstract: Objective To analyze the expression of secreted aspartyl proteinases (Sap) in human vaginal infection in vivo. Methods Vaginal swabs were collected from 9 healthy volunteers and 20 patients with vaginal candidiasis, and the expression of Sap1-6 was evaluated by reverse-transcriptase polymerase chain reaction using specific primer sets. Results The Sap2 and Sap5 were the most common genes expressed in the patients with vaginal candidiasis. The expression Sap3 and Sap4 was detected in all subjects. All six Sap genes were simultaneously expressed in some patients with vaginal candidiasis. Conclusion The data shows that the Sap genes may be involved in the pathogenesis of vaginal candidiasis.

Key words: Candidiasis,vulvovaginal, Candida albicans, Aspartic endopeptidases