中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (4): 217-219.

• 论著 • 上一篇    下一篇

催乳素与促生长素对体外培养系统性红斑狼疮患者单一核细胞产生细胞因子的影响

黄雯, 冯树芳, 徐金华, 李峰   

  1. 复旦大学附属华山医院皮肤科 上海 200040
  • 收稿日期:2004-03-15 出版日期:2005-04-15 发布日期:2005-04-15

Effect of Prolactin and Growth Hormone on the Secretion of Th1/Th2 Type Cytokines in the Patients with Systemic Lupus Erythematosus

HUANG Wen, FENG Shu-fang, XU Jin-hua, LI Feng   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2004-03-15 Online:2005-04-15 Published:2005-04-15

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摘要： 目的 研究催乳素、促生长素对系统性红斑狼疮(SLE)患者Th1/Th2型细胞因子的影响。方法 对SLE患者和正常人对照的淋巴细胞进行体外培养,并测定细胞因子。结果 在自分泌情况下,SLE活动期患者白介素6(IL-6)、白介素10(IL-10)的分泌量显著增高,而干扰素γ(IFN-γ)的分泌量低于静止期和正常人,但差异无统计学意义。经促生长素和催乳素干预后,活动期SLE患者IL-6、IL-10分泌量显著高于自分泌量和正常人对照组。IFN-γ分泌量经促生长素和催乳素干预后,与自分泌量相比,活动期患者无显著升高。促生长素对SLE患者IL-6、IL-10、IFN-γ分泌量的影响与催乳素的影响差异无统计学意义。结论 SLE患者不但存在Th2型细胞因子增高,而且促生长素和催乳素均能刺激Th2型细胞因子进一步增高,且刺激能力相当,并呈正相关。

关键词: 红斑狼疮,系统性, 生长激素(人), 催乳素, 白细胞介素6, 白细胞介素10, 干扰素Ⅱ型

Abstract: Objective To study the effect of growth hormone (GH) and PRL (prolactin) on the secretion of Th1/Th2 type cytokines in the patients with systemic lupus erythematosus (SLE). Methods The secretion of cytokines in PBMCs stimulated by GH and PRL was detected by ELISA in vitro. Results The results showed that the secretion levels of IL-6 and IL-10 from PBMCs of the SLE patients in active stage were higher than those of the normal controls, and the secretion level of IFN-γ was lower than that of SLE patients in resting stage and the normal controls, but it is of no statistical significance. The secretion levels of IL-6 and IL-10 were significantly increased in PBMC stimulated by GH and PRL, but GH and PRL had no effect on the secretion of IFN-γ. There was no difference on the secretion level of IL-6, IL-10 and IFN-γ when the PBMC was stimulated by GH and PRL. Conclusion GH and PRL might play an important role in the pathogenesis of SLE.

Key words: Lupus erythematosus, systemic, Somatropin, prolactin, Interleukin-6, Interleukin-10, Interferon type Ⅱ