中华皮肤科杂志 ›› 1995, Vol. 28 ›› Issue (3): 144-145.

• 论著 • 上一篇    下一篇

酶联免疫吸附法检测血清中抗解脲支原体抗体

邹先彪1, 张国威2   

  1. 1. 解放军第三O四医院皮肤科, 北京100037;
    2. 第三军医大学新桥医院皮肤科
  • 收稿日期:1994-05-22 修回日期:1994-09-19 出版日期:1995-06-15 发布日期:1995-06-15

Detection of Antibody to Ureaplasma Urealyticum by ELISA

Zou Xianbiao1, Zhang Guowei 2   

  1. Department of Dermatology, Affiliated Xinqiao Hospital, Third Military Medical University, Chongqing 630038
  • Received:1994-05-22 Revised:1994-09-19 Online:1995-06-15 Published:1995-06-15

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摘要： ELISA间接法检测临床感染解脲支原体的患者血清抗体,与代谢抑制法相比,22例培养阳性的标本,其血清抗体检出率,ELISA法检出21例,代谢抑制法检出22例,其一致率为95%.在81例培养阳性的血清标本中,93.8%有IgG、30.8%有IgM,而在81例培养阴性的血清标本中,2.5%有IgG,3.7%有IgM.用ELISA不能检出型特异性抗体,这种交叉反应可能是由于在解脲支原体中有同源性抗原存在。

关键词: 解脲支原体, 抗-抗体

Abstract: In the paper,a modified ELISA was first developed for the detection of antibody to Ureaplasma urealyticum(UU) in clinical specimens. Antibody to UU in serum of patient was detected by indirect ELISA. In comparison with metabolism inhibition test(MIT), of 22 UU culture-positive individuals, 21 were positive by ELISA and 22 by the MIT with an overall agreement of 95.5%. IgG antibodies to UU were found in 93.8% and IgM in 30.8% of 81 UU culture-positive patients,whereas IgG antibodies in 2.5% and IgM in 3.7% of the UU culture-negative group by ELISA. Meantime,serovartype-specific antibody could not be detected by ELISA with type-specific antigen, the cross-reaction might be due to the presence of homogeneous antigens among UU species.

Key words: Ureaplasma urealyticum, Anti-antibodies