中华皮肤科杂志 ›› 1995, Vol. 28 ›› Issue (6): 381-383.

• 论著 • 上一篇    下一篇

圆形糠秕孢子菌菌丝相培养研究

王文岭1, 朱一元2   

  1. 1. 安徽医科大学附属医院皮肤科, 230022;
    2. 白求恩国际和平医院皮肤科
  • 收稿日期:1995-03-24 修回日期:1995-06-12 出版日期:1995-12-15 发布日期:1995-12-15
  • 基金资助:
    安徽省科研基金资助课题

Study on the Culture of Mycelial Phase of Pityrosporum Orbiculare

Wang Wenting1, Zhu Yiyuan 2   

  1. Department of Dermatology, Affiliated Hospital of Anhui Medical University, Hefei 230022
  • Received:1995-03-24 Revised:1995-06-12 Online:1995-12-15 Published:1995-12-15

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摘要： 为了探讨体外圆形糠秕孢子菌(P.orb)菌丝相形成的某些条件和间接地了解花斑癣发病的部分原因,我们利用含胆固醇半固体培养基初步培养出该菌菌丝相,研究了该菌菌丝相形成与培养基中胆固醇浓度、琼脂浓度之间的关系.结果表明,P.orb在含胆固醇1.0g/dl、琼脂0.8g/dl的培养基上培养4～6天后开始有菌丝形成,以后菌丝渐多,10～12天达高峰.多为芽生菌丝,呈球拍状.长约8μm～16μm.簇集性分布.0.25g/dl是胆固醇在体外诱导P.orb菌丝相形成的最低阈值浓度.在0.25g/dl～1.0g/dl范围内,胆固醇浓度愈高,P.orb菌丝愈多.超过1.0g/dl后,胆固醇浓度再增加,菌丝增加不明显.相对高湿度(0.8g/dl琼脂)较适于P.orb菌丝形成.实验结果提示,花斑癣在夏季发病或复发有可能与夏季反复出汗、汗液蒸发后体表胆固醇浓度等改变有关.

关键词: 马拉色霉菌属, 花斑癣

Abstract: In order to investigate some requisite conditions for the formation of mycelial phase of Pityrosporum orbiculare (P.orb) and understand indirectly the pathogenesis of tinea versicolor, we used a semisolid medium containing cholesterol 1.0g/dl and agar 0.8g/dl, and obtained the mycelial phase of P.orb.Then we studied the relationship between the formation of the mycelial phase and the concentration of cholesterol or agar in the medium.The results showed that when P.orb were cultured on the medium, the hyphae began to grow on the 4-6th day, increased gradually, and peaked on the 10-12th day.The hyphae were mostly blastohyphae, 8-16μm in length and in the shape of racket,and distributed in clustes.It was found that 0.25g/dl was the minimum concentration of cholesterol to induce the mycelial phase in vitro.Within the range of 0.25-1.0g/dl the higher the concentration was, the more the hyphae were.When the concentration exceeded 1.0g/dl, the hyphae did not increase markedly.Relative high moisture (0.8g/dl of agar in the medium) was suitable for the production of the hyphae.The results suggest that the occurrence and recurrence of tinea versicolor in summer probably relate to the change of choleserol concentration on the surface of the skin after the evaporation of sweat.

Key words: Malassezia, Tinea versicolor