中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (1): 43-44.

• 论著 • 上一篇    下一篇

基底细胞癌p53基因缺失和突变检测

高玲, 王椿森, 李家文, 张德美, 杨琦   

  1. 同济医科大学附属协和医院皮肤科, 武汉430022
  • 收稿日期:1995-01-11 修回日期:1995-04-21 出版日期:1996-02-15 发布日期:1996-02-15

Detection of Deletion and Mutation of P53 Gene in Basal Cell Carcinoma

Gao Ling, Wang Chunsheng, Li Jiawen   

  1. Union Hospital, Tongji Medical University, Wuhan 430022
  • Received:1995-01-11 Revised:1995-04-21 Online:1996-02-15 Published:1996-02-15

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摘要： 我们应用新近建立的基因突变检测技术聚合酶链反应-单链构象多态性,检测了12例基底细胞癌(BCC)和5例正常对照的石蜡包埋标本。发现BCCp53基因丢失和突变率为42%(5/12),正常对照未见异常。其结果提示p53基因的异常改变可能是BCC发生、发展的重要环节。

关键词: 基底细胞癌, p53

Abstract: We used recently developed technique,polymerase chain reaction-single stranded conformation polymorphism(PCR-SSCP) for detection of gene mutation,and paraffin embedded tissues of 12 patients with basal cell carcinoma and 5 controls were used for examinotion.It was found that the deletion and mutation rate of p53 gene in basal cell carcinoma was 42% (5/12),whereas no deletion and mutation were observed in 5 normal tissue specimens.Our results indicate that the abnormality of p53 gene is an important event in carcinogenesis and evolution of BCC.

Key words: Carcinoma,basal cell, p53