中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (1): 27-30.

• 论著 • 上一篇    下一篇

前列腺素E1对角朊细胞和真皮纤维母细胞增殖及细胞因子产生的影响

张建中1, 丸山幸治2, 金子史男2   

  1. 1. 北京医科大学第一医院皮肤科;
    2. 日本福岛医科大学皮肤科
  • 收稿日期:1995-01-03 修回日期:1995-09-26 出版日期:1996-02-15 发布日期:1996-02-15

Effects of Prostaglandin E1 on Cell Proliferation and Cytokine Production in Human Keratinocytes and Dermal Fibroblasts

Zhang Jianzhong1, Kohji Maruyama2, Fumio Kaneko2   

  1. Department of Dermatology, The First Hospital, Beijing Medical University, Beijing 100034
  • Received:1995-01-03 Revised:1995-09-26 Online:1996-02-15 Published:1996-02-15

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摘要： 人工合成前列腺素E1(PGE1)治疗皮肤创伤及慢性溃疡有明显疗效。为了探讨其治疗机理,我们用体外细胞培养方法,将表皮角朊细胞及真皮纤维母细胞培养中加入PGE1,用MTT法测定细胞增殖,用酶联免疫吸附(ELISA)法测定各种细胞因子的产生。结果表明角朊细胞和纤维母细胞所产生的细胞因子种类存在一定差异。角朊细胞主要产生IL1α、IL8、转化生长因子α及血小板源生长因子,而纤维母细胞则主要产生IL6和IL8。PGE1可明显促进纤维母细胞IL6产生,也轻度促进IL8的产生,但对角朊细胞的细胞因子产生则无明显影响。同样PGE1对纤维母细胞增殖有明显促进作用,但对角朊细胞的增殖无明显影响,提示PGE1、对皮肤溃疡和刨伤的治疗作用可能主要是通过对纤维母细胞的作用实现的。

关键词: 前列腺素E1, 角朊细胞, 纤维母细胞

Abstract: Prostaglandin E1(PGE1) has been proven to be effective in the treatment of chronic skin ulcers and wounds.To elucidate the possible mechanism involved,normal human keratinocytes and dermal fibroblasts were cultured with PGE1.Cell proliferation was measured by MTT assay and cytokine production by enzyme-linked immunosobent assay (ELISA).The results revealed that keratinocytes produced IL-1α,IL-8,TGFα and PDGF,while dermal fihroblasts produced IL-6 and IL-8,suggesting dif-ferent kinds of cytokine production between keratinocytes and dermal fibroblasts.PGE1 promoted IL-6 and IL-8 production in dermal fibroblasts but not in keratinocytes.Moreover,PGE1 promoted cell prolif-eration in dermal fibroblasts but not in keratinocytes,suggesting that dermal fibroblasts are more sensi-tive than keratinocytes to PGE1 and that the therapeutic effects of topical PGE1 on skin ulcer or wound may mainly be mediated by its effects on dermal fibroblasts.

Key words: Prostaglandin E1, Keratinocyte, Fibroblast