中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (5): 291-293.

• 论著 • 上一篇    下一篇

含HPV16 E7的病毒样颗粒诱导小鼠产生特异性CTL反应

程浩1, 叶俊1, 周健2   

  1. 1. 浙江大学医学院附属邵逸夫医院皮肤科, 杭州310016;
    2. 澳大利亚昆士兰大学免疫和癌症研究中心
  • 收稿日期:2004-05-06 出版日期:2005-05-15 发布日期:2005-05-15
  • 基金资助:
    国家自然科学基金资助项目(30271190)

CTL Response Induction in Mice Immunised with Chimeric BPVL1/HPV16 E7 Virus-Like Particles

CHENG Hao1, YE Jun1, ZHOU Jian2   

  1. Department of Dermatology, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou 310016, China
  • Received:2004-05-06 Online:2005-05-15 Published:2005-05-15

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摘要： 目的 探讨含BPVL1/HPV16E7嵌合型乳头瘤病毒样颗粒(VLPs)在动物小鼠体内的免疫学特性。方法 将HPV16E7基因分为(a)、(b)、(c)3段,与BPVL1连接后表达的BPVL1/HPV16E7(a)、(b)、(c)嵌合型VLP分别免疫小鼠C57BL/6J,对其淋巴结淋巴细胞进行细胞毒性T淋巴细胞反应(CTL)分析。结果 接受嵌合型VLPBPVL1/HPV16E7(b)免疫的小鼠淋巴结淋巴细胞可以引发很强的对C-2细胞(HPV16E7aa47-59转染的EL-4细胞)的特异性CTL反应,但不能对EL-4细胞产生细胞毒性作用。结论 含BPVL1/HPV16E7嵌合型VLP作为抗原输送系统致敏小鼠T淋巴细胞并引发抗原特异性CTL反应,可能为HPV疫苗的设计提供一个新手段。

关键词: 病毒粒子, T淋巴细胞,细胞毒性, 乳头状瘤病毒,人, 基因,病毒

Abstract: Objective To study immunological property of chimeric BPVL1/HPV16 E7 virus-like particles (VLPs) in mice. Methods HPV16 E7 gene was cut into three fragments, ligated to BPVL1 sequence, respectively, and then expressed as chimeric VLPs. The ability of the chimeric VLPs to stimulate in vivo cytotoxic response was analysed with lymphocytes taken from mice C57BL/6J lymph nodes. Results A strong CTL response against C-2 cells (HPV16 E7 aa47-59 transfected EL-4 cell) was induced in mice immunised with chimeric VLPs BPVL1/HPV16 E7(b). Furthermore, no CTL response was detected against EL-4 cells in immunized mice. Conclusions Chimeric BPVL1/HPV16 E7 VLPs, serving as antigens, can activate mouse T lymphocytes and elicit a strong antigen-specific CTL response. Chimeric BPVL1/HPV16 E7 VLPs could be used as an efficient antigen delivery system, and might provide a novel strategy for HPV16 vaccine design.

Key words: Papillomavirus, human, Genes, viral, Virion, T-lymphocytes, cytotoxic