中华皮肤科杂志 ›› 1996, Vol. 29 ›› Issue (6): 399-401.

• 论著 • 上一篇    下一篇

幼儿急疹病因学的研究

陈斌姚, 季晓辉, 周瑶玺   

  1. 江苏省人民医院皮肤科, 南京 210029
  • 收稿日期:1995-12-04 修回日期:1996-06-26 出版日期:1996-12-15 发布日期:1996-12-15
  • 基金资助:
    国家自然科学基金

Studyonthe Etiology of Exanthema Subitum

Chen Bin, Yao Kun, Ji Xiaohui   

  1. Department of Dermatology, Jiangsu Province Hospital, Nanjing 210029
  • Received:1995-12-04 Revised:1996-06-26 Online:1996-12-15 Published:1996-12-15

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摘要： 为了探讨幼儿急疹致病病毒,采用病毒分离培养、电镜、血清学试验、聚合酶链反应(PCR)对11例幼儿急疹患儿进行了研究.结果从7例患儿体内分离出一种病毒,此病毒能感染人脐血单一核细胞,产生典型细胞病变.电镜下可见感染细胞中疱疹样病毒颗粒;血清学试验证明分离株与HHV-6GS株间存在抗原一致性;PCR结果表明该分离株HHV-6特异性DNA阳性.

关键词: 猝发疹, 人疱疹病毒

Abstract: In order to explore the causal virus for exanthema subitum(ES),11 patients with ES were studied by virus isolation,electronmicroscopy,serological test and PCR.One virus strain was isolated from seven patients.The isolate could infect human umbilical cord blood mononuclear cels,and the cytopathic effects characterized by large baloon-like cels were observed after infection.Electronmi-croscopy of infected cells in ultrathin sections showed enveloped virus particles which were morphologicaly similar to herpes group virus.Serol ogical test further demonstrated that the isolates were antigenically related to HHV-6GS strain.HHV-6 specific DNA was detected in all the isolates by PCR.All the results confirmed that theisolated virus was HHV-6.In addition,paired sera colected from ES patients during acute and convalescent phases were tested for the antibody against new viralisolates.The antibody titre of convalescent phase was 4 times higher than that of the acutephase,and the convalescent seracould neutralize the infectivity of the new isolates.The results confirmed that the new isolated HHV-6 was the causalagent for ES.

Key words: Exanthema subitum, Herpesvirus hominis