中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (3): 167-169.

• 论著 • 上一篇    下一篇

聚合酶链反应和直接免疫荧光法检测沙眼衣原体感染的方法比较

王宏伟, 王家璧, 刘跃华, 王晓峰, 郑占才   

  1. 中国医学科学院、中国协和医科大学北京协和医院皮肤科, 北京100730
  • 收稿日期:1996-07-22 修回日期:1997-01-07 出版日期:1997-06-15 发布日期:1997-06-15

Comparison of Polymerase Chain Reaction and Direct Fluorescent Assay for Diagnosis of Chlamydia Trachomatis Infections

Wang Hongwei, Wang Jiabi, Liu Yuanhua   

  1. Department of Dermatology, Peking Union Medical College Hospital(PUMCH), Beijing 100730
  • Received:1996-07-22 Revised:1997-01-07 Online:1997-06-15 Published:1997-06-15

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摘要： 沙眼衣原体所致泌尿生殖道感染是最常见的性传播疾病之一。对110例患者进行聚合酶链反应(PCR)与直接免疫荧光法(DFA)检测,比较二者在临床检测中的优缺点。PCR引物为主要外膜蛋白基因片段,阳性65例(59.1%),阴性45例(40.9%).DFA阳性43例(39.1%),阴性67例(60.9%),PCR和DFA阳性率有显着性差异(P<0.01),病程长短对PCR阳性率无明显影响(P>0.05),病程长短对DFA阳性率有显着影响(P<0.01),病程长者DFA阳性率低。PCR特别适用于临床中病程长且DFA阴性患者。

关键词: 沙眼衣原体, 聚合酶链反应, 直接免疫荧光法

Abstract: Chlamydia trachomatis infection is one of the prevalent sexaully transmitted diseases(STD) of the urogenital tract. A total of 110 clinical specimens from patients attending STD clinics were detected by using polymerase chain reaction (PCR), and were compared with direct fluorescent assay (DFA). Oligonucleotide primers were designed to amplify a fragment within the conserved region of a major outer membrane protein(MOMP) gene of Chlamydia trachomatis, the positive rate of PCR was 59.1% (65/110), and negative rate 40.9% (45/110). The positive rate of DFA was 39.1% (43/110), and negative rate were 60.9% (67/110). The Chlamydia trachomatis positive rates were significantly different between PCR and DFA (P<0.01). The relationship between the course of disease and the detection rates by PCR and DFA was analyzed. The positive rate of PCR was not related to the course of disease (P>0.05),but the positive rate of DFA was related to the course of the disease, the longer the course of disease(>2 months) the lower the positive rate of DFA (P<0.01). It is the authers′ opinion that PCR is useful for those patients with long course of disease and negative DFA result.

Key words: Chlamydia trachomatis, Polymerase chain reaction, Direct fluorescent assay