中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (4): 240-242.

• 论著 • 上一篇    下一篇

某些皮肤肿瘤的不同角蛋白和丝聚合蛋白及桥粒斑蛋白的表达

李广志, 白兆震, 何春涤, 王雅坤, 陈洪铎   

  1. 中国医科大学附属第一临床学院皮肤科, 沈阳 110001
  • 收稿日期:1996-05-20 修回日期:1996-11-05 出版日期:1997-08-15 发布日期:1997-08-15

Expression of Different Keratins, Filagrin and Desmoplakins in Some Skin Tumors

Li Guangzhi, Bai Zhaozhen, He Chundi   

  1. Department of Dermatology, No.1 Hospital of China Medical University, Shenyang 110001
  • Received:1996-05-20 Revised:1996-11-05 Online:1997-08-15 Published:1997-08-15

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摘要： 为了探讨某些皮肤肿瘤的不同角蛋白和丝聚合蛋白及桥粒斑蛋白(desmoplakins)的表达情况,用抗表皮(AE)系列单抗、连续冰冻切片法及ABC免疫组织化学技术对几种皮肤肿瘤进行了研究。结果:①Bowen病、蕈样肉芽肿(MF)、基底细胞癌(BCC)、鳞状细胞癌(SCC)的病损处表皮基底层角朊细胞与AE1反应减弱或消失,棘层、颗粒层角朊细胞可与AE1反应。SCC、Paget病、Bowen病的瘤细胞可与AE1反应。②疣状痣、脂溢性角化病颗粒层角朊细胞不与AE3反应。③Paget病基底层角朊细胞不与AE14反应,而角化棘皮瘤、脂溢性角化病可见棘层、颗粒层角朊细胞与AE14反应。AE14可与BCC癌巢本身周边的基底样细胞反应,而不与SCC的瘤细胞反应。④AE21不与色素痣颗粒层角朊细胞反应,也不与BCC和SCC的瘤细胞反应,但BCC和SCC的瘤细胞均与AE22反应。并探讨了这种异常表达的可能机理及其临床意义。

关键词: 角蛋白, 抗体, 单克隆, 皮肤肿瘤

Abstract: In order to investigate the expression of different keratins, filagrin, and desmoplakins in some skin tumors, a panel of anti-epidermal monoclonal antibodies (AE), serial sections of frozen biopsy specimens and ABC immunohistochemical technique were employed. Results are as follows: ①The reaction of AE1 with basal keratinocytes decreased or disappeared in Bowen's disease, mycosis fungoides (MF), basal cell carcinoma (BCC), and squamous cell carcinoma (SCC), while the prickle and granular keratinocytes reacted with AE1 and the tumor cells of SCC, Paget's disease and Bowen's disease reacted with AE1. ②AE3 did not react with the granular keratinocytes in verrucous neavus and seborrheic keratosis. ③AE14 did not react with the basal keratinocytes in Paget's disease, while it reacted with the prickle, granular keratinocytes in keratoaeanthoma and seborrheic keratosis. AE14 reacted with the peripheral basaloid cells of BCC cancer nest itself, while it did not react with the tumor cells of SCC. ④AE21 did not react with the granular keratinocytes in pigmented neavus and the tumor cells of BCC and SCC, but the tumor cells of both BCC and SCC reacted with AE22. The possible mechanism and clinical significance of the abnormal expression were also discussed.

Key words: Keratin, Antibodies, monoclonal, Skin neoplasms