中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (5): 302-305.

• 论著 • 上一篇    下一篇

卡氏枝孢霉与阿耶罗枝孢瓶霉的随机扩增DNA多态性研究

席丽艳1, Kaoru Okada2, 泽香代子3, 前林行雄2, 宫治诚2, 吴绍熙1, 福岛和贵2   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042;
    2. 日本千叶大学真核微生物研究中心;
    3. 日本东京情报大学
  • 收稿日期:1996-11-04 修回日期:1997-02-27 出版日期:1997-10-15 发布日期:1997-10-15

Characterization of Cladosporium carrionii and Cladophialophora ajelloi by Random Amplification of Polymorphic DNA Assay

Xi Liyan1, K. Okada2, K. Takizawa3   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College Peking Union Medical College, Nanjing 210042
  • Received:1996-11-04 Revised:1997-02-27 Online:1997-10-15 Published:1997-10-15

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摘要： 研究卡氏枝孢霉与阿耶罗枝孢瓶霉的DNA多态性,了解两菌在DNA型别与形态学及菌种来源地域的相互关系.采用溴苯提取法提取DNA,以随机扩增DNA多态性方法对来自五个国家的21株分离株(19株卡氏枝孢霉,2株阿耶罗枝孢瓶霉)进行研究.结果:①10个随机引物筛选出3个具有较好DNA扩增的引物.②19株卡氏枝孢霉的DNA带型具有较大的遗传相似性,阿耶罗枝孢瓶霉的DNA带型与卡氏枝孢霉略有不同,但与中国株具有较大相似性(85%的遗传相似性).③卡氏枝孢霉的遗传相似性与菌株的来源地域关系密切,同一国家的菌株具有相似的DNA带型,位于树状图的同一树组中.结论:随机扩增DNA多态性研究发现卡氏枝孢霉具有一定的种内差异,其DNA带型与菌株的来源地域关系密切,阿耶罗枝孢瓶霉的DNA带型与某些株卡氏枝孢霉更相似.本研究方法简便、快速,可用于流行病学及基因学特征的研究.

关键词: 卡氏枝孢霉, 阿耶罗枝孢瓶霉, 随机扩增DNA多态性

Abstract: In order to investigate the DNA polymorphism of Cladosporium carrionii and Cladophialophora ajelloi,and to find the relationship of DNA patterns with morphology and geographic origin,the total DNA was extracted by using benzyl bromide method,and random amplification of polymorphic DNA(RAPD) assay was used to the study of genetic similarity(GS) of 21 isolates from five countries(19 of Cladosporium carrionii,2 of Cladophialophora ajelloi).Results:(1)Of ten random primers used,three gave good reaction.(2) The DNA fragment patterns of 19 isolates of Cladosporium carrionii showed high genetic similarity,but a little difference in comparison with that of 2 isolates of Cladophialophora ajelloi.The RAPD patterns of two isolates showed high genetic similarity with most Chinese isolates of Cladosporium carrionii(GS:85%).(3) The genetic similarity of Cladosporium carrionii are closely related to the geographic origin,the DNA patterns of isolates from the same country showed high similarity and located in the same group of dendrogram.RAPD assay can be used for the study of epidemiology and genetic characterization as a simple,rapid method.

Key words: Cladosporium carrionii, Cladophialophora ajelloi, Random amplification of polymorphic DNA