中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (6): 386-388.

• 论著 • 上一篇    下一篇

白介素1α及白介素6对角朊细胞与真皮成纤维细胞相互作用的影响

张建中1, 丸山幸治2, 金子史男2   

  1. 1. 北京医科大学人民医院皮肤科 100044;
    2. 日本福岛医科大学皮肤科
  • 收稿日期:1996-08-22 修回日期:1997-01-20 出版日期:1997-12-15 发布日期:1997-12-15

Role of Interleukin-1α and Interleukin-6 in the Interaction Between Keratinocytes and Dermal Fibroblasts

Zhang Jianzhong1, Kohji Maruyama2, Fumio Kaneko2   

  1. Department of Dermatology, People’s Hospital, Beijing Medical University, Beijing 100044
  • Received:1996-08-22 Revised:1997-01-20 Online:1997-12-15 Published:1997-12-15

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摘要： 为了探讨细胞因子在表皮与真皮相互作用中所起的作用,用体外细胞培养的方法研究了白介素1α(IL-1α)及白介素6(IL-6)对角朊细胞与真皮成纤维细胞之间相互作用的影响以及前列腺素E1(PGE1)的调节作用.IL-1α主要由角朊细胞产生,而IL-6则主要由成纤维细胞产生.来自成纤维细胞的培养上清液有较强的促进角朊细胞增殖能力.来自角朊细胞的培养上清液也有一定促进成纤维细胞增殖能力.IL-1α和PGE1可促进成纤维细胞IL-6的产生.抗IL-6抗体可抑制成纤维细胞培养上清液对角朊细胞的促生长活性.以上结果说明IL-1α和IL-6可能是介导表皮-真皮相互作用的重要因子.

关键词: 白介素1α, 白介素6, 角朊细胞, 纤维母细胞

Abstract: An in vitro study was performed to elucidate the possible role of interleukin-1α(IL-1α) and IL-6 in the interaction between keratinocytes and dermal fibroblasts and the regulatory effects of prostaglandin E1(PGE1). The results showed that IL-1α was mainly produced by keratinocytes and IL-6 mainly by dermal fibroblasts. Conditioned media from dermal fibroblasts stimulated the proliferation of keratinocytes, and to a less extent vice versa. PGE1 and recombinant IL-1α enhanced IL-6 production in dermal fibroblasts. However, recombinant IL-6 had little effects on IL-1α production in keratinocytes. Anti IL-6 antibody inhibited the proliferation of keratinocytes grown in fibroblast derived conditioned media, suggesting that IL-6 is a major factor responsible for the growth-promoting effect of the conditioned media. These results suggest that IL-1α and IL-6 are important mediators in the interaction between epidermis and dermis.

Key words: IL-1α, IL-6, Keratinocyte, Fibroblast