中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (1): 32-34.

• 论著 • 上一篇    下一篇

肿瘤坏死因子受体Ⅱ在郎格罕细胞迁移中的作用

蔡钦朝1, 王秉鹤2   

  1. 1. 安徽中医学院基础部, 合肥230038;
    2. 南京医科大学第一附属医院
  • 收稿日期:1997-01-05 修回日期:1997-08-01 出版日期:1998-02-15 发布日期:1998-02-15

Role of TNF Receptor in Migration of Langerhans Cells

Cai Qinchao1, Wang Binghe2   

  1. Department of Basic Medical Sciences, Anhui College of Traditional Chinese Medicine, Hefei 230038
  • Received:1997-01-05 Revised:1997-08-01 Online:1998-02-15 Published:1998-02-15

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摘要： 目的 应用肿瘤坏死因子 (TNF)受体基因敲除突变型鼠,探讨 TNFα介导郎格罕细胞(LC)向局部淋巴结迁移、引发免疫应答过程中,其两种膜受体:TNF受体和TNF受体 所起的信号转导作用。方法 以免疫荧光标记及流式细胞分析技术分别观察TNF受体缺失鼠和经抗TNFα中和性抗体预处理以阻断其体内TNFα生物学效应的TNF受体缺失鼠在FITC接触致敏后,LC自皮肤向淋巴结的迁移情况,并与野生型鼠对照。结果 经致敏后,TNF受体缺失鼠淋巴结内出现的FITC携带细胞(证实为LC)数量与野生型鼠无明显差异;而经抗TNFα抗体预处理的TNF受体 缺失鼠引流淋巴结内LC数量明显少于未经 TNFα抗体预处理的对照组。结论 TNF受体在介导LC迁移中并非必需;在TNF受体 缺失情况下,TNF受体 的信号转导对LC的迁移可能发挥关键作用。

关键词: 受体Ⅱ,肿瘤坏死因子, 郎格罕细胞

Abstract: Objective To elucidate the role of signal transduction TNF receptor I(TNF R1) and TNF receptor (TNF R2) in Langerhans cells (LC) migration in immuno-responses,TNF R1gene-knock-out mutant mice were used in this study.Methods The LC migration from skin to regional lymph nodes after FITC sensitizatioon in both the TNF R1-deficient mice and the TNF R1-deficient mice pretreated with neutralizing antibody to TNFα were examined by im munolabelling and FCM,and TNFR1+/+ mice were used as control. Results Following FITC sensitization there was no significant difference between the number of FITC+ cells(LC) found in lym ph nodes from TNF R1-deficient mice,and from the wild type (TNFR1+/+) mice,and the number of LC found in the draining lymph nodes fromanti-TNFα antibody pretreatment TNF R1-deficient mice was significantly lower than that of the mice not pretreated by anti-TNFα antibody. Conclusion It suggests that TNFR1is not necessary in mediating LC migration and TN-F R2 might be crucial in signal transduction in TNF R1-deficient mice.

Key words: ReceptorⅡ, Tumor necrosis factor, Langerhans cells, Signal transduction