中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (3): 152-155.

• 论著 • 上一篇    下一篇

解脲支原体对七种抗菌药物的敏感性测定及其与相关耐药基因检测的比较

骆丹1, 黄澍杰2, 谢礼豪2, 汪宁3, 朱文元1, 徐文严4   

  1. 1. 南京医科大学第一附属医院皮肤科 210009;
    2. 广东省江门市皮肤病防治所;
    3. 南京铁道医学院流行病教研室;
    4. 中国医学科学院、中国协和医科大学皮肤病研究所
  • 收稿日期:1997-06-24 修回日期:1997-12-08 出版日期:1998-06-15 发布日期:1998-06-15

Study on Susceptibility of Ureaplasma Urealyticum to Antimicrobial Agents and tetM Gene Detection

Luo Dan1, Huang Shujie2, Xie Lihao2   

  1. Department of Dermatology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029
  • Received:1997-06-24 Revised:1997-12-08 Online:1998-06-15 Published:1998-06-15

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摘要： 目的 以肉汤稀释法对解脲支原体(Uu)进行7种抗菌药物的敏感性测定,并与聚合酶链反应(PCR)方法检测四环素耐药决定子的结果进行比较,探讨其临床及实验意义.方法 42个Uu分离株经三次传代成纯培养物后接种于96孔板小孔中,药液倍比稀释成64μg/ml～0.06μg/ml,37℃培养,加药组以72小时后仍不出现培养基由黄→红色变化的最小药物浓度为该药的最小抑菌浓度(MIC).根据tetM基因序列设计引物,采用PCR方法进行扩增,并对PCR产物进行酶切消化反应.结果 四环素抗菌活性较差,MIC≥8μg/ml以上的中度敏感有12株,且美满霉素、强力霉素的MIC也多达8μg/ml与4μg/ml.尚未发现对三类抗菌药同时耐药的Uu分离株.42个Uu株的tetM阳性检测率为71.4%,除1例外均出现预期酶解片段.结论 ①Uu分离株对四环素族药敏性较差,临床上经四环素类反复治疗难以阴转,并有症状的Uu感染病例可换用另外两类抗支原体药物;②载有tetM的Uu株可能成为耐药株,应结合MIC进行监测及随访.

关键词: 解脲支原体, 微生物敏感性试验

Abstract: Objective To determine the susceptibility of Ureaplasma urealyticum(Uu) to 7 kinds of antimicrobial agents,to compare the result with tetracycline resistant determinant(tetM) detected by PCR technique.Methods Forty two Uu isolates were subcultured for 3 generations and then cultured with drugs with a series of concentrations from 64μg/ml～0.06μg/ml.MIC was determined when the color of culture medium did not change from yellow to red in 72h culture.The tetM determinant in the isolates was detected by PCR with specific primers,and PCR products were digested by Taq 1 restrictive endonuclease.Results The antimicrobial activity of tetracyclines to Uu was low.No Uu strains resistant simultaneously to 3 groups of antibiotics were found in the study.Detection rate of tetM was 71.4% with correspondent digested fragments.Conclusion The susceptibility of Uu isolates to tetracycline group was low,but higher to macrolides and fluoroquinolones.Uu strains carrying tetM determinant might become drug resistant ones which should be monitored and followed up with MIC determination.

Key words: Ureaplasma urealyticum, Microbial sensitivity tests