中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (5): 285-287.

• 论著 • 上一篇    下一篇

白念珠菌粘附素的分离纯化及其在介导与宿主细胞粘附中的作用

刘晓红1, 廖万清2, 胡惠民3, 姚志荣2, 王君松1, 赵子卿1   

  1. 1. 济南军区第八十八医院皮肤科, 泰安271000;
    2. 第二军医大学附属长征医院;
    3. 第二军医大学生化教研室
  • 收稿日期:1997-09-22 修回日期:1997-12-16 出版日期:1998-10-15 发布日期:1998-10-15
  • 基金资助:
    国家自然科学基金

Isolation and Purification of Adhesin of Candida Albicans and its Effect on Adherence to Host Cells

Liu Xiaohong1, Liao Wangqing2, Hu Huiming3   

  1. Department of Dermatology, Eighty eighth Hospital of Jinan Military Area Command, Taian 271000
  • Received:1997-09-22 Revised:1997-12-16 Online:1998-10-15 Published:1998-10-15

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摘要： 目的 从分子水平探讨白念珠菌(简称白念)与宿主细胞的粘附机制。方法和结果 用非离子去垢剂辛基吡喃葡萄糖苷提取白念孢子壁蛋白质,然后制备纤连蛋白Sepharose 4B亲和柱,运用亲和层析技术从上述提取液中分离纯化纤连蛋白粘附素,在非还原状态进行十二烷基磺酸钠聚丙烯酰胺凝胶电泳(SDSPAGE),得到表观分子量60000和105000的二条蛋白带。为了弄清此蛋白在白念与宿主细胞粘附中的作用,把人颊粘膜上皮细胞与白念孢子共同振荡孵育2小时,显微镜下测定上皮细胞表面粘附白念的数目,白念与上皮细胞的粘附百分率为44.7±6.28,上皮细胞经纤连蛋白粘附素预处理后,粘附百分率为28.3±6.18,P<0.01.结论 纤连蛋白粘附素介导了人颊粘膜上皮细胞与白念的粘附。

关键词: 白念珠菌, 纤连蛋白, 粘附

Abstract: Objective To investigate the adherence mechanism of Candida albicans to host cells in molecular level. Methods and Results Yeast cell wall protein of Candida albicans were extracted, and purified fibronectin (Fn) adhesin was obtained from yeast cell wall extract. Fn adhesin was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE), the molecular weights of Fn adhesin were 60kd and 105kd. In order to find out the effect of the protein on adherence to host cells, human buccal epithelial cells and Candida albicans cells were incubated at 37℃ for 2 hours on a rotator, and yeasts adhering to the surfaces of epithelial cells were assayed microscopically. The adherence rate of C.albicans to buccal epithelial cells was 44.7±6.28%, and after treating the epithelial cells with Fn adhesin, the adherence rate reduced to 28.3±6.18%, P<0.01. Conclusion The results indicate Fn adhesin could mediate adherence of Candida albicans to epithelial cells.

Key words: Candida albicans, Fibronectin, Adhere