中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (1): 3-5.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者外周血单一核细胞中Th1型细胞因子的表达

陈志强, 李子仁, 范江, 岳晓玉, 徐文严   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:1998-05-20 修回日期:1998-08-27 出版日期:1999-02-15 发布日期:1999-02-15
  • 基金资助:
    高等院校博士学科点专项科研基金!(9502084)资助课题

Th1-Type Cytokines Expression in the Peripheral Mononuclear Cells of the Patients with Systemic lupus Erythematosus

CHEN Zhiqiang, LI Ziren, FAN Jiang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1998-05-20 Revised:1998-08-27 Online:1999-02-15 Published:1999-02-15

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摘要： 目的 探讨系统性红斑狼疮(SLE)患者中Th1型细胞因子表达的情况.方法 用逆转录聚合酶链反应(RTPCR)法检测了SLE患者外周血单一核细胞(PBMC)中白介素2(IL2)mRNA表达(41例)和γ干扰素(IFNγ)mRNA表达(31例)的水平.结果 与正常人相比,活动期SLE患者中IL2表达水平降低者占78.0%,IFNγ表达水平降低者占83.9%.活动期SLE组PBMC中IL2和IFNγmRNA表达的平均水平也降低(P均<001).结论 SLE患者中Th1型细胞因子的表达下调,可能在发病机制中起一定作用.

关键词: 红斑狼疮,系统性, 白细胞介素2, 干扰素Ⅱ型, RNA,信使, 逆转录-聚合酶链反应

Abstract: Objective To investigate the mRNA expression of Th1 type cytokines in SLE patients. Methods Applying RT-PCR technique to semiquantitatively analyse the IL-2 mRNA expression in PBMCs from 41 SLE patients and IFN-γ mRNA expression in PBMCs from 31 SLE patients. Results Compared with normal controls,the SLE patients with decreased expression of IL-2 accounted for 78.0% of the SLE patients in active stage,and those with decreased expression of IFN-γ accounted for 83.9%,the mean levels of IL-2 and IFN-γ expression in SLE group were significantly decreased as well (P<0.01,both). Conclusion The Th1 type cytokine expression of SLE patients in active stage is down regulated,which may play a role in the pathogenesis of SLE.

Key words: Lupus erythematosus,systemic, Interleukin-2, Interferon typeⅡ, RNA,messenger, Polymerase chain reaction