中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (4): 252-253.

• 病例报告 • 上一篇    下一篇

C57BL6小鼠毛发周期动物模型的建立

杨淑霞1, 马圣清1, 钟志红1, 秦俭1, 李志新2, 武玲慎1   

  1. 1. 北京医科大学第一附属医院皮肤科 100034;
    2. 北京医科大学中西医结合教研室
  • 收稿日期:1998-08-01 修回日期:1998-11-28 出版日期:1999-08-15 发布日期:1999-08-15
  • 基金资助:
    宝洁公司资助课题

Establishment of Hair Cycle Animal Model with C57BL6 Mice

YANG Shuxia1, MA Shengqing1, ZHONG Zhihong1   

  1. Department of Dermatology, The First Hospital, Beijing Medical University, Beijing 100034
  • Received:1998-08-01 Revised:1998-11-28 Online:1999-08-15 Published:1999-08-15

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摘要： 目的 为研究毛发周期,本实验建立了适合的动物模型.方法 用C57BL6小鼠为动物模型,对25只小鼠用松香/蜡(1:1)混合物拔毛诱导其毛发自休止期进入生长期,每天观察皮肤色泽变化和毛囊组织学改变.结果 拔毛处皮肤颜色于第5～6d由粉红色(休止期)变成黑色(生长期),第17～19d又经灰色(退行期)变成粉红色(休止期).组织学显示,小鼠在拔毛后第5～6d处于生长期第Ⅳ亚期,第17～19d由生长期进入退行期,第20d以后,均处于休止期.松香/蜡混合物拔毛可诱导C57BL6小鼠同步进入新的毛发生长周期,并且小鼠皮肤颜色的变化和组织学上毛发周期时相的变化是相符的.结论 用C57BL6小鼠作为毛发周期动物模型是可行的.

关键词: 毛发, 小鼠,近交C57BL6

Abstract: Objective To established an animal model for studying the hair cycle. Methods Using C57BL6 mice as animal model, hair shafts of 25 mice were depilated by wax/resin mixture to induce the hair follicles from telogen to anagen. Skin color and histological changes were observed daily. Results After depilation, the skin color of those mice turned from pink (telogen) to black (anagen) in day 5～6 and turned from gray (catagen) to pink (telogen) in day 17～19. Histologically, anagen (substage IV) took place in day 5～6, changed to catagen in day 17～19, and entered telogen in day 20 after depilation.It showed that depilation by wax/resin mixture could induce C57BL6 mice from telogen to highly synchronized hair growth cycle. The changes of skin color were consistent with the histological changes of hair cycle. Conclusion C57BL6 mice could be used as an animal model for further studies on hair cycle.

Key words: Hair Mice, inbred C57BL6