中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (5): 333-335.

• 论著 • 上一篇    下一篇

100株临床分离致病酵母体外药敏试验分析

章强强, 苏逸丹, 李莉, 王家俊   

  1. 上海医科大学华山医院皮肤科, 200040
  • 收稿日期:1999-12-09 出版日期:2000-10-15 发布日期:2000-10-15

Antifungal Susceptibility Testing of 100 Clinical Isolates of Pathogenic Yeasts

ZHANG Qiangqiang, SU Yidan, LI Li   

  1. Huashan Hospital, Shanghai Medical University, Shanghai 200040
  • Received:1999-12-09 Online:2000-10-15 Published:2000-10-15

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摘要： 目的 评价临床致病性酵母对抗真菌药物敏感性及耐药性。方法 根据美国国家临床实验室标准化委员会M27-A微量稀释法对临床致病性酵母作了氟康唑、二性霉素B、5-氟胞嘧啶、伊曲康唑及酮康唑5种抗真菌药物敏感性测定。结果 65株念珠菌属的MIC范围为:氟康唑0.125～64μg/mL,95.38%(62/65)敏感,2株耐药,占3.08%;伊曲康唑0.03～2μg/mL,63.08%(41/65)敏感,3株耐药,占4.62%;5-氟胞嘧啶0.125～32μg/mL,93.84%(61/65)敏感,2株耐药,占3.08%;二性霉素B0.06～2μg/mL,96.92%(63/65)敏感,2株耐药,占3.08%;酮康唑0.03～0.5μg/mL,全部敏感(65/65)。35株新生隐球菌临床分离株对5种抗真菌药物的MIC范围分别为:氟康唑2～64μg/mL,伊曲康唑0.25～1μg/mL,5-氟胞嘧啶0.25～64μg/mL,二性霉素B0.03～1μg/mL,酮康唑0.125～1μg/mL。结论 美国国家临床实验室标准化委员会M27-A微量稀释法具有操作简便,实用性强,结果稳定,重复性好的优点。临床致病性酵母及其不同的菌株对5种常用抗真菌药物的敏感性存在差异。

关键词: 念珠菌属, 新型隐球菌, 微生物敏感性试验

Abstract: Objective To evaluate the sensitivity and resistance of pathogenic yeasts isolated from patients to antifungal agents. Methods The in vitro susceptibilities of pathogenic yeasts isolated from clinical patients to amphotericin B(AmB),5-flucytosine(5-FC),itraconazole(ICZ),ketoconazole(KCZ) and fluconazole(FCZ) were determined by NCCLS M27-A method. Results MIC ranges of 65 strains of Candida spp.were: FCZ 0.125～64μg/mL, 95.38% (62/65) were sensitive,2 strains(3.08%) were resistant; ICZ 0.03～2μg/mL, 63.08%(41/65) sensitive,3 strains(4.62%) were resistant; 5FC 0.125～32μg/mL, 93.84%(61/65) sensitive, 2 strains(3.08%) resistant; AmB 0.06～2μg/ml,96.92% (63/65) were sensitive,2 strains(3.08%) were resistant; KCZ 0.03～0.5μg/mL,all strains(65/65) were sensitive. For 35 strains of Cryptococcus neoformans, the MIC were: FCZ 2～64μg/mL, ICZ 0.25～1μg/mL, 5FC 0.25～64μg/mL, AmB 0.03～1μg/ml,KCZ 0.125～1μg/mL. Conclusions NCCLS M27-A micro-dilution method is reliable, practical, reproducible and easy to use in the clinical laboratory. The sensitivity of various species and strains of fungi to the above mentioned antifungal agents is different.

Key words: Candida, Cryptococcus neoformans, Microbial sensitivity tests