中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (6): 398-400.

• 论著 • 上一篇    下一篇

体外雷公藤内酯醇对白介素1和白介素2生成和活性的抑制

李新宇, 郑家润, 唐美育, 徐兰芳   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:1999-12-07 出版日期:2000-12-15 发布日期:2000-12-15
  • 基金资助:
    国家自然科学基金资助(39870901)

Effects of Tripterygium wilfordii on the Production and Activities of IL-1 and IL-2 in vitro

LI Xinyu, ZHENG Jiarun, TANG Meiyu   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1999-12-07 Online:2000-12-15 Published:2000-12-15

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摘要： 目的 研究雷公藤内酯醇(T0)对白介素1、2生成、活性的药效学作用,并与环孢素A(CyA)作对比研究,以考核这两个细胞因子作为药物作用靶位及建立药效学方法的依据。方法 大鼠腹腔巨噬细胞-大鼠胸腺细胞系统和大鼠脾细胞-小鼠IL-2依赖细胞系统,[甲基3H]胸腺嘧啶核苷掺入法。结果 T0和CyA都能抑制大鼠巨噬细胞IL-1和大鼠脾细胞IL-2的生成,并呈量-效关系。T0对IL-1和IL-2活性亦有抑制作用,而CyA在药理浓度下无此作用。结论 T0和CyA均为IL-1和IL-2生成强有力的抑制剂,T0的效价高于CyA。对IL-1和IL-2的活性,两药作用不同。

关键词: 白细胞介素1, 白细胞介素2, 雷公藤内酯, 环孢菌素

Abstract: Objective To investigate the effects of the compound T0 extracted from Tripterygium wilfordii, a traditional Chinese herb, in comparison with those of cyclosporin A(CyA), on down-regulating the expression and activities of IL-1 and IL-2. Methods The cellular reactive systems of peritoneal macrophage (rat)-thymocytes (rat), and spleen cells (rat)-CTLL-2 (mouse) were set up. The technique of 3H-TdR incorporation was applied. Results The production of IL-1 and IL-2 were significantly inhibited by T0 and CyA. The activities of IL-1 and IL-2 were down-regulated by T0,rather than by CyA. Conclusion T0 and CyA are potent inhibitors of the production of IL-1 and IL-2. Effects of T0 on the activities of IL-1 and IL-2 are different from those of CyA.

Key words: Interleukin-1, Interleukin-2, Triptolide, Cyclosporine