中华皮肤科杂志 ›› 2001, Vol. 30 ›› Issue (1): 28-30.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者外周血单一核细胞CD40/CD40配体mRNA的表达

弓娟琴, 陈志强, 贾国泉, 胡兹嘉, 李文忠   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京 210042
  • 收稿日期:2000-02-17 出版日期:2001-02-15 发布日期:2001-02-15
  • 基金资助:
    卫生部科学研究基金资助课题(981041)

Expression of CD40 and CD40 Ligand in the Peripheral Blood Mononuclear Cells of the Patients with Systemic Lupus Erythematosus and Its Clinical Significance

GONG Juanqin, CHEN Zhiqiang, JIA Guoquan   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:2000-02-17 Online:2001-02-15 Published:2001-02-15

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摘要： 目的 探讨CD40及其配体(CD40L)在系统性红斑狼疮(SLE)发病机制中的作用及临床意义.方法 应用逆转录－聚合酶链反应(RT-PCR)检测34例SLE患者外周血单一核细胞(PBMC)中CD40和CD40配体mRNA的表达水平.结果 活动期SLE患者PBMC中CD40配体的阳性表达率为88.24%(30/34),明显高于正常人对照组(50.00%,15/30),差异有非常显著性(P<0.01);活动期SLE患者PBMC中CD40配体mRNA表达的平均水平(0.6970±0.1214)亦明显高于正常人对照组(0.2684±0.0795),差异有非常显著性(P<0.01).活动期SLE患者PBMC中CD40的阳性表达率和CD40mRNA的表达水平与对照组相比差异均无显著性(P>0.05).结论 CD40配体的异常表达可能在SLE疾病活动和发展过程中起重要作用,检测SLE患者PBMC中CD40配体的表达水平可作为疾病的活动性指标之一.

关键词: 红斑狼疮,系统性, 抗原,CD40, CD40配体

Abstract: Objective To investigate the role of CD40 and CD40 ligand (CD40L)in the pathogenesis of systemic lupus erythematosus (SLE)and its clinical significance. Methods Applying RT-PCR technique to semiquantitatively analyze CD40 and CD40L mRNA expression in PBMC from 34 SLE patients. Results Compared with normal controls,the positive CD40L expression accounted for 88.24% of active SLE patients(P<0.001); the mean level of CD40L mRNA expression in active SLE group was markedly increased as well (P<0.0001). However,there were no statistical differences in positive rate of CD40 and the average level of CD40 mRNA between the two groups(P >0.05,both). Conclusions The abnormal expression of CD40L might play an important role in the pathogenesis of SLE,and the level of CD40L might be used as one the indices for active SLE.

Key words: Lupus erythematosus,systemic, Antigens,CD40, Ligand,CD40