中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (5): 355-357.

• 论著 • 上一篇    下一篇

申克孢子丝菌随机扩增DNA多态性研究

廉翠红1, 金礼吉2, 刘晓明1, 安利佳2, 杨国玲1, 林熙然1   

  1. 1. 大连医科大学附属第一医院皮肤科, 116011;
    2. 大连理工大学化工学院生物工程系
  • 收稿日期:2000-10-12 出版日期:2001-10-15 发布日期:2001-10-15
  • 基金资助:
    辽宁省教委资助课题(990221069)

Characterization of Sporothrix schenckii by Random Amplification of Polymorp hic DNA Assay

LIAN Cuihong1, JIN Liji2, LIU Xiaoming1   

  1. Department of Dermatology, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China
  • Received:2000-10-12 Online:2001-10-15 Published:2001-10-15

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摘要： 目的 了解申克孢子丝菌的基因学特征,研究DNA分型与菌种来源和临床表现的关系.方法 以随机扩增DNA多态性方法对来源于不同地区及不同临床类型的24株申克孢子丝菌进行DNA多态性分析.结果 ①筛选出3个具有较好扩增片段的引物,即引物OPAA11:5'-ACCCGACCTG-3',OPD18:5'-GAGAGCCAAC-3',OPB07:5'-GGTGACGCAG-3'.②24株菌的DNA带型不完全相同,具一定遗传变异性.③不同菌株同一引物扩增均见一共有DNA片段.④播散型DNA带型与皮肤淋巴管型不同.结论 随机扩增DNA多态性方法可用于申克孢子丝菌的DNA分型.其带型与菌株的地区来源和临床表现有关.

关键词: 随机扩增多态DNA技术, 申克孢子丝菌

Abstract: Objective To investigate the DNA polymorphism of S. schenckii and to find the ralationship between DNA patterns,geographicareas,and clinical manifestations.Methods The total DNA was extracted with hexadecyltrimethylammonium bromide.Random amplification of polymorphic DNA(RAPD)assay was used to study DNA typing in 24 strains of S. schenckii which were collected from different areas and from different clinical types.Results ①Of seven random primers used,three primers(OPAA11、OPD18 and OPB07) gave good reactions,the sequences of which were 5'-ACCCGACCTG-3',5'-GAGAGCCAAC-3',5'-GGT-GACGCAG-3',respectively.②The RAPD patterns of the 24 isolates were not identical,showing certain degrees of hereditary variation.③Different isolates showed a common conserved DNA band with the same primer.④There were different genotypes between different clinical types.Conclusion According to RAPD typing of S. schenckii,the DNA band type is related to its geographic origin and clinical manifestation.

Key words: Random amplified polymorphic DNA technique, Sporothrix schenckii