中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (1): 31-33.

• 论著 • 上一篇    下一篇

应用抑制性消减杂交技术筛选和克隆斑秃脱发相关基因

宋志强, 郝飞, 钟白玉, 向明明, 刁庆春, 刘荣卿   

  1. 第三军医大学西南医院皮肤科, 重庆400038
  • 收稿日期:2001-05-18 出版日期:2002-02-15 发布日期:2002-02-15
  • 基金资助:
    国家自然科学基金资助课题(30070375);重庆市科委重点攻关课题(2001-8-4)

Screening and Cloning the Genes Related to Alopecia Areata by Suppression Subtractive Hybridization

SONG Zhiqiang, HAO Fei, ZHONG Baiyu, XIANG Mingming, DIAO Qingchun, LIU Rongqing   

  1. Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
  • Received:2001-05-18 Online:2002-02-15 Published:2002-02-15

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摘要： 目的 筛选和克隆斑秃脱发相关基因。方法 分别分离斑秃脱发区和非脱发区毛乳头后,应用抑制性消减杂交比较脱发区和非脱发区毛乳头基因表达的差异,对脱发区毛乳头特异表达的基因进行克隆、测序和同源性分析。结果 建立了斑秃脱发区毛乳头cDNA消减文库,并成功地克隆到一条斑秃毛乳头特异表达的基因片段,同源性检索证实其为一条自身抗原基因。结论 所建立的消减文库可能含有斑秃发病相关基因,所克隆的到自身抗原基因在斑秃发病中的作用值得进一步研究。

关键词: 斑秃, 毛乳头, 抑制性消减杂交

Abstract: Objective To screen and clone the genes related to alopecia areata. Methods Dermal papillae of lesional and non-lesional follicles were separated from alopecia areata scalp respectively. Suppression subtractive hybridization was used to investigate the difference of expressed genes in dermal papillae of lesional (tester) and non-lesional (driver) follicles, and the differentially expressed genes in dermal papillae of lesional follicles were cloned and sequenced. Results A subtractive library of dermal papillae of lesional follicles from alopecia areata was established. A differentially expressed gene in dermal papillae of lesional follicles was successfully cloned and proved to be an autoantigen gene. Conclusions The subtractive library may contain the differentially genes related to alopecia. The autoantigen gene related to alopecia areata need to be further investigated.

Key words: Alopecia areata, Papilla, dermal, Suppression subtractive hybridization