中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (2): 131-133.

• 论著 • 上一篇    下一篇

常见致病性念珠菌的PCR-RFLP鉴定研究

余进1, 冀朝晖2, 李若瑜1   

  1. 1. 北京大学第一医院皮肤科、北京大学真菌和真菌病研究中心 100034;
    2. 北京大学基础医学院生物化学与分子生物学系 100034
  • 收稿日期:2001-08-15 出版日期:2002-04-15 发布日期:2002-04-15
  • 基金资助:
    卫生部临床学科重点项目资助课题(97020214)

Identificantion of Common Pathogenic Candida Species by PCR-RFLP Analysis

YU Jin1, JI Zhaohui2, LI Ruoyu1   

  1. Department of Dermatology, Peking University First Hospital, Beijing 100034, China
  • Received:2001-08-15 Online:2002-04-15 Published:2002-04-15

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摘要： 目的 研究常见致病性念珠菌的分子鉴定方法,为深部念珠菌病的分子诊断奠定基础。方法 对常见9种致病性念珠菌的11株标准株和39株临床株的核糖体DNA内转录间隔区进行聚合酶链反应扩增,对扩增产物进行MspⅠ、HaeⅢ和DdeⅠ三种内切酶的酶切分析。结果 9种念珠菌聚合酶链反应扩增后产生5种不同分子量的条带,扩增产物经MspⅠ、DdeⅠ和HaeⅢ酶切后分别产生8种、5种和4种特异性带型。结论 聚合酶链反应-限制性内切酶片段长度多态性方法在鉴定常见的致病性念珠菌中稳定、可靠、特异,是传统方法的有益补充。

关键词: 念珠菌属, 聚合酶链反应, 多态性,限制性片段长度

Abstract: Objective To study the molecular identification method of Candida species and lay the foundation of molecular diagnosis for deep candidiasis. Methods PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 11 type strains of 9 pathogenic Candida spp. and 39 clinical Candida isolates, and the products were digested with three types of restriction endonucleases (MspⅠ,DdeⅠand HaeⅢ). Results Five patterns of products with different molecular weights were obtained after amplification from 9 Candida spp., and the products were digested with MspⅠ, DdeⅠand HaeⅢ, producing 8, 5 and 4 unique band patterns, respectively. Conclusion PCR-RFLP is reliable, specific and useful for the identification of common pathogenic Candida species.

Key words: Candida, Polymerase chain reaction, Polymorphism, restriction fragment length