中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (5): 272-373.

• 论著 •    下一篇

隐球菌性脑膜炎患者脑脊液隐球菌活性的研究

顾菊林, 温海, 廖万清   

  1. 第二军医大学长征医院皮肤科, 上海200003
  • 收稿日期:2002-04-18 出版日期:2002-10-15 发布日期:2002-10-15

Study on the Viability of Cryptococcus in Cerebrospinal Fluid of Patients with Cryptococcal Meningitis

GU Julin, WEN Hai, LIAO Wanqing   

  1. Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Received:2002-04-18 Online:2002-10-15 Published:2002-10-15

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摘要： 目的 对隐球菌性脑膜炎(隐脑)患者脑脊液进行隐球菌活性研究。方法 对脑脊液标本进行电镜观察、动物接种和中性红染色检查。结果 透射电镜观察见在治疗早期,多数标本菌体较为完整,常能见到出芽现象;在治疗后期,胞浆水肿、荚膜结构排列紊乱常见。通过对连续多次常规真菌培养不生长而镜检阳性的脑脊液标本进行动物接种,结果全部感染。中性红染色检查见多数标本中均存在一定数量的深血红色菌体。结论 上述方法可以对隐脑患者脑脊液隐球菌菌体活性进行动态观察,作为临床疗效监测的重要指标。

关键词: 脑膜炎,隐球菌性, 隐球菌属

Abstract: Objective To evaluate the viability of Cryptococcus in cerebrospinal fluid of patients with cryptococcal meningitis.Methods Electron microscopy,animal inocul ation and neutral red staining of the cere-brospinal fluid specimens were empl oyed.Results Transmission electron microscopy revealed intact cells and budding cells of cryptococcus which appeared frequently during the early treatment.Edema of cytoplasm and disar-rangement of structure of capsule were often found during the later therapy.All mice inoculated experimentally with the cerebrospinal fluid specimens were positive on direct examination but negative in routine culture were infected.A definite number of deep blood-red fungal cells were observed in many specimens.Conclusion These findings add a new approach for dynamic studying of the viability of Cryptococcus in cerebrospinal fluid of patients with Crypto-coccal meningitis and provide an important parameter for evaluating therapeutic effect.

Key words: Meningitis,Cryptococcal, Cryptococcus