中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (5): 355-357.

• 论著 • 上一篇    下一篇

用聚合酶链反应进行红色毛癣菌种内分型

李民1, 王家俊1, 章强强1, 李莉1, 兰和奎2, 任大明2   

  1. 1. 上海复旦大学附属华山医院皮肤科真菌室, 200040;
    2. 复旦大学遗传所遗传工程国家重点实验室
  • 收稿日期:2001-12-10 出版日期:2002-10-15 发布日期:2002-10-15

Strain Typing by PCR in Trichophyton rubrum

LI Min1, WANG Jiajun1, ZHANG Qiangqiang1, LI Li1, LAN Hekui2, REN Daming2   

  1. Laboratory of Mycology, Department of Dermatology, Huashan Hospital, FuDan University, Shanghai 200040, China
  • Received:2001-12-10 Online:2002-10-15 Published:2002-10-15

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摘要： 目的 建立一种快速且可重复的方法应用于红色毛癣菌的菌株区分。方法 PCR扩增20株红色毛癣菌核糖体基因的非转录间隔区内的两个串联重复亚单位trs-1、trs-2,克隆两段PCR产物,用PGEM-T载体构建重组质粒载体进行目的基因测序。结果 特异性扩增trs-1和trs-2区产生菌株特征的条带图。两个串联重复亚单位的基因测序结果说明了PCR指纹图差异的原因。结论 这种PCR方法产生的特征性的指纹图提供了一种快速、稳定的分子分型方法,可应用于红色毛癣菌感染的流行病学和致病性的进一步研究。

关键词: 发癣菌属, 真菌学分型技术

Abstract: Objective To develop a rapid and reproducible m ethod in strain identification for T.rubrum.Methods Two novel tandem repeat subelements(TRSS),trs-1 and trs-2,located in the T.rubrum rDNA non-transcribed spacer(NTS) were amplified from 20 strains of T.rubrum.The PCR products were cloned into seq uencing vector PGEM-T for sequencing.Results Specific amplification of trs-1 and trs-2 produced strain-characteristic band patterns(PCR types),the sequence from trs-1 and trs-2 also supported this finding.The trs-1 sequence variation was found in a strain of T.rubrum which can grow at 37℃.Conclusion The characteristic fingerprints generated by this PCR assay provide a rapid,stable molecular typing method to study the epidemiology and pathogenicity of T.rubrum infection.

Key words: Trichophyton, Mycological typing techniques