中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (6): 445-448.

• 论著 • 上一篇    下一篇

皮肤淋巴瘤与EB病毒潜伏感染的关系及病毒亚型的分析

徐子刚1, 赵佩云1, 马圣清2, 岩月启氏3, 金子史男3   

  1. 1. 首都医科大学附属北京儿童医院皮肤科 北京 100045;
    2. 北京大学第一医院皮肤科;
    3. 日本福岛医科大学皮肤科
  • 收稿日期:2001-11-29 出版日期:2002-12-15 发布日期:2002-12-15

Association of Epstein-Barr Virus Latent Infection with Cutanous Lymphoma and Analysis of Virus Subtypes

XU Zigang1, ZHAO Peiyun1, MA Shengqing2, IWATSUKI K3, KANEKO F3   

  1. Department of Dermatology, Beijing Children's Hospital, Affiliated to Capital University of Medical Sciences, Beijing 100045, China
  • Received:2001-11-29 Online:2002-12-15 Published:2002-12-15

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摘要： 目的 探讨EB病毒潜伏感染及其亚型与皮肤淋巴瘤的相关性.方法 标本来源于41例淋巴瘤患者的皮肤组织和38例健康成人咽部洗漱液.采用原位杂交、PCR、巢式PCR、Southern杂交方法检测EB病毒及其亚型.通过Southern杂交对5例自然杀伤/T细胞淋巴瘤病例中EB病毒末端重复序列进行分析.结果 8例自然杀伤/T细胞淋巴瘤、4例皮下脂膜炎型淋巴瘤、1例自然杀伤/T淋巴细胞增生症、1例血管免疫母细胞恶性淋巴瘤和1例组织细胞样淋巴瘤EB病毒原位杂交和PCR结果均阳性.38例正常人中有14例咽部携带有EB病毒.15例蕈样肉芽肿、5例Sézary综合征病例中,EB病毒原位杂交和EB病毒BMRF1基因PCR扩增均为阴性.除1例淋巴细胞增生症患者为EB病毒亚型1和2双重感染外,其它病例均为亚型1感染.正常人群咽部携带亚型1EB病毒.5例自然杀伤/T细胞淋巴瘤与EB病毒末端重复序列特异性探针杂交后,均只产生一条杂交带.结论 EB病毒潜伏感染与皮肤自然杀伤/T细胞淋巴瘤及皮下脂膜炎型淋巴瘤相关,但与蕈样肉芽肿和Sézary综合征无明显相关性.肿瘤细胞中的EB病毒与正常人群咽部所携带的病毒亚型相同,均为亚型1.肿瘤细胞单克隆增殖发生于EB病毒感染之后,此结论支持EB病毒在自然杀伤/T细胞淋巴瘤中的致病作用.

关键词: 疱疹病毒4型,人, 淋巴瘤

Abstract: Objective To investigate the association of Epstein-Barr virus (EBV) infection with certain forms of cutaneous lymphomas and analyze the subtypes of infected virus. Methods Clinical specimens were obtained from skin biopsies of 41 patients with EBV-associated cutaneous lymphomas and throat washing of 38 healthy adults. EBV positivity was established by detecting EBV-encoded small nuclear RNAs (EBERs) with in situ hybridization, and confirmed by polymerase chain reaction (PCR). The subtype of EBV was identified by nested PCR and Southern hybridization. Five cases were subjected to clonality analysis of EBV by determi ning the terminal repeats. Results EBV was identified in 8 of 9 patients with NK /T cell lymphoma, 4 of 9 with subcutaneous panniculitis T-cell lymphoma, 1 of 1 with NK/T cell lymphocytosis, 1 of 1 with angioimmunoblastic lymphadenopathy-l ike T-cell lymphoma, and 1 of 1 with histiocytoid lymphoma, as indicated by pos itive results of EBERs in situ hybridization and PCR amplifying EBV BMRF1 gene.No evidence of EBV latent infection was shown in cases of mycosis fungoides or Sézary syndrome. Subtype 1 virus was classified in all EBV positive samples. Dual infections with EBV sutypes 1 and 2 were found in a case of NK/T cell lymphocytosis. Meanwhile, EBV was detected in 14 of 38 specimens from throat washing of healthy individuals and subtype 1 was identified. Clonality analysis of EBV showed a single band in DNA hybridization which indicated that clonal proliferation of the neoplastic cells contained EBVs with identical terminal repeats. Conclusions EBV latent infection is associated with NK/T cell lymphomas and subcutanous T-cell lymphomas, but not related to mycosis fungoides or Sézary syndrome. EBV subtype 1 is prevalent in both lymphoma patients and healthy individuals. It i s suggested that monoclonal cellular proliferation occur after EBV infection, which supports an etiologic role of EBV in NK/T cell lymphoma.

Key words: Herpesvirus 4, human, Lymphoma