中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (1): 10-12.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者血清催乳素水平和外周血单一核细胞催乳素受体的表达

李锋1, 徐金华1, 冯树芳1, 陆云2, 邓守贞2   

  1. 1. 复旦大学附属华山医院皮肤科, 上海200040;
    2. 复旦大学附属华山医院核医学科, 上海200040
  • 收稿日期:2002-01-07 出版日期:2003-01-15 发布日期:2003-01-15
  • 基金资助:
    国家自然科学基金资助(39770688)

Serum Prolactin Level and Prolactin Receptor Expression on Peripheral Blood Mononuclear Cells in Patients With Systemic Lupus Erythematosus

LI Feng1, XU Jin-hua1, FENG Shu-fang1, LU Yun2, DENG Shou-zhen2   

  1. Department of Dermatology, Affiliated Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2002-01-07 Online:2003-01-15 Published:2003-01-15

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摘要： 目的 探讨系统性红斑狼疮(SLE)患者血清催乳素(PRL)水平和外周血淋巴细胞的催乳素受体(PRLR)的表达与SLE活动性的关系。方法 应用时间分辨免疫发光试验(TrFIA)测定血清PRL,用SLEDAI指数评估了113例SLE患者的病情活动性,并用Logistic多元回归研究临床表现、免疫学指标与高PRL血症的关系;采用放射性受体配基结合试验(RLBA)及RT-PCR研究外周血淋巴细胞PRLR变化。结果 ①63例SLE活动期患者血清PRL水平(14.45±7.25μg/L)显著高于50例静止期患者(11.79±8.08μg/L)和28例正常人对照组(7.08±6.26μg/L);②79.37%的活动期患者血清PRL水平在9~51.2μg/L;③113例SLE患者血清PRL水平与dsDNA抗体、SLEDAI活动指数呈线性正相关;④Logistic多元回归研究发现蛋白尿、低C3和高dsDNA抗体滴度与高PRL血症相关;⑤24例SLE活动期患者外周血单一核细胞(PBMC)的PRLR的特异性结合率(SB,5.03%±2.51%)、总结合率(TB,15.34%±6.98%)和mRNA的表达水平(0.85±0.45)均显著高于22例静止期患者(SB4.18±2.26,TB14.03±6.54%,mRNA0.58±0.43)和15例正常人对照组(SB1.62%±1.05%,TB8.19%±1.47%,mRNA0.20±0.13)。结论 SLE患者血清PRL轻度升高,PBMC的PRLRmRNA高表达以及相应特异性结合率的增加与SLE的病情活动性相关。

关键词: 红斑狼疮,系统性, 催乳素, 受体,催乳素

Abstract: Objective To study the association between serum prolactin(PRL)level,prolactin receptor(PRLR)expression on peripheral blood mononuclear cells and the disease activity in the patients with systemic lupus erythematosus(SLE).Methods Serum PRL level was measured by time-resolved fluoroimmunoassay(TrFIA)in 113 patients of SLE and in 28 gender-and-age matched control subjects,SLEDAI index was estimated.It was also investigated by logistic multiple regression analysis that the association between clinical manifestations,immunologic parameters,anti-dsDNA antibody titers and hyperprolactinemia in 113 patients of SLE.The specific binding(SB)rate of peripheral blood lymphocyte PRLR was measured by radioactive binding ligand assay(RLBA)and the mRNA expression of PRLR by reverse transcription polymerase chain reaction(RT-PCR)in 24 active SLE patients,22 inactive SLE patients and 15 gender-and-age matched control subjects.Results The serum PRL levels of 63 active patients were much higher than those of 50 inactive patients and 28 control subjects.The serum PRL levels ranged 9~51.2μg/L in 79.3% of active patients.It was also found that PRL level was in positive linear correlation with the titer of anti-dsDNA antibody.The concentration of interleukin 2 receptors in hyperprolactinemia group was higher than that in normal group.It was shown that proteinuria,low levels of complement 3 and high titers of anti-dsDNA antibody were associated with hyperprolactinemia by logistic multiple regression analysis.The SB rate of PRL receptor was 5.03±2.51%(x±s),the total binding rate(TB)was 15.4±6.98% in 24 active patients with SLE.The SB rate of active patients was much higher than that of 22 inactive patients(SB4.18±2.26%,TB rate 14.03±6.54%)and that of 15 gender-and-age matched control subjects(SB1.62±1.05%,TB8.19±1.47%).The mRNA expression of PRLR in active patients(x±s,0.85±0.45)was much higher than in inactive patients(0.58±0.43)and that in control subjects(0.20±0.13).Conclusion The slightly increased serum level of PRL,high expression of PRLR and the increased specific binding rate are associated with the disease activity of SLE.

Key words: Lupus erythematosus,systemic, Prolactin, Receptor,prolactin