中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (2): 70-72.

• 论著 • 上一篇    下一篇

皮肤T细胞淋巴瘤的T细胞受体基因克隆性重排

汪晨1,2, 桑建利1, 张海江1   

  1. 1. 北京师范大学生命科学学院 100875;
    2. 中日友好医院皮肤科
  • 收稿日期:2002-02-19 出版日期:2003-02-15 发布日期:2003-02-15
  • 基金资助:
    教育部重点实验室访问学者基金资助(教育部科技司[2000]123号)

Analysis of T-cell receptorγgene rearrangement for cutaneous T-cell lymphomas

WANG Chen1,2, Sang Jian-li1, Zhang Hai-jiang1   

  1. College of Life Sciences, Beijing Normal University, Beijing 100875, China
  • Received:2002-02-19 Online:2003-02-15 Published:2003-02-15

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摘要： 目的 研究皮肤T细胞淋巴瘤(CTCL)早期诊断。方法 利用PCR方法,设计TCRVγ1-8、Vγ9、Jγ1/γ2特异引物,分析24例各类型CTCL,2例可疑CTCL及4例非特异性红皮病患者的33份皮肤和6份淋巴结石蜡标本的TCRγ-GR克隆性,30例炎性皮疹石蜡标本为阴性对照。结果 39份标本中38-GR克隆性,提示他们为“克隆性皮炎”,需长期随访。结论 用PCR发现早期CTCL的TCRγ-GR克隆性,为诊断提供依据。

关键词: 淋巴瘤, T细胞, 皮肤, 基因, T细胞受体γ

Abstract: Objective To study the early diagnosis of cutaneous T-cell lymphoma(CTCL).Methods DNA from39formalin-fixed,paraffin-embedded tissues of 24 cases of CTCL,2 cases of dubious lymphoma,and4cases with non-specific erythroderma,was amplified by polymerase chain reaction(PCR),with special primers of TCR Vγ1-8,Vγ9,and Jγ1/γ2 to analyze T-Cell receptorγgene clonal rearrangement(TCR-GR clonal).The specimens from30patients with non-specific dermatitis were taken as negative control.Results Clonal of TCR Vγ1-8 was demonstrated in38of39paraffin-embedded specimens.Vγ9-GR was shown in 37 of 39 specimens TCRγ-GR was shown especially in 13 patients with MF of early stage,2 patients with dubious CTCL,and4patients with non-specific erythroderma,as well as in 6 of 30 patients with histiologically nonspecific dermatitis which suggested that these 6 cases were "clonal dermatitis".Conclusion Clonal TCR γ-GR may be detected in patients with early CTCL,even when the histologic findings are not unequivocally diagnostic.

Key words: Lymphoma, T-cell, cutaneous, Genes, T-cell receptor gamma