中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (6): 329-331.

• 论著 • 上一篇    下一篇

脂质体鬼臼毒素混悬液在大鼠表皮及真皮的分布规律初探

曾抗1, 张三泉1, 江彬彬1, 孙乐栋1, 李建华1, 马晓冬3, 朱晓亮1, 李国锋2   

  1. 1. 广州第一军医大学南方医院皮肤科 510515;
    2. 第一军医大学中心实验室 510515;
    3. 广州第一军医大学南方医院药学部 510515
  • 收稿日期:2002-03-05 出版日期:2003-06-15 发布日期:2003-06-15
  • 基金资助:
    国家自然科学基金资助项目(39970680)

Epidermal and Dermal Distribution After Topical Application of Liposome Podophyllotoxin Suspension in Rats

ZENG Kang1, ZHANG San-quan1, JIANG Bin-bin1, SUN Le-dong1, LI Jian-hua1, MA Xiao-dong3, ZHU Xiao-liang1, LI Guo-feng2   

  1. Department of Dermatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China
  • Received:2002-03-05 Online:2003-06-15 Published:2003-06-15

PDF (PC)

130

赞

摘要： 目的 探讨脂质体鬼臼毒素在大鼠皮肤各层的分布规律。方法 观察组大鼠涂抹0.5%脂质体鬼臼毒素混悬液,对照组涂抹0.5%鬼臼毒素酊剂,分别在涂药1、2、4、6、12及24h取皮肤标本,常规石蜡切片,采用激光共聚焦显微成像仪(CLSM)扫描,观察单位面积鬼臼毒素的荧光量。结果 鬼臼毒素24h荧光值曲线下面积(AUC),表皮层中观察组是对照组的1.5倍,真皮层中是2.3倍;对照组在涂药2h后,表皮和真皮的荧光量即达到峰值(分别为1585.52/μm2和2005.66/μm2),在4h后荧光量迅速降低;而观察组在4h前,表皮和真皮中的荧光量较低,6h后逐渐增高,在12h达峰值(分别为(750.28/μm2和1073.08/μm2)。结论 脂质体制剂使鬼臼毒素在皮肤中缓慢释放,并持续较长时间。

关键词: 脂质体, 鬼臼毒素, 投药, 皮肤

Abstract: Objective To study the distribution pattern of liposome podophyllotoxin(LP)in rat skin.Methods The rats were divided into two groups:0.5% liposome podophyllotoxin suspension was applied to LP group,0.5% podophyllotoxin tincture was applied to control group.The skin specimens were obtained 1、2、4、6、12 and 24h after drug application,the amount of fluorescent stain was observed under confocal laser scanning microscope and converted to the values of area under the curve(AUC).Results The epidermal AUC of fluorescent amount in LP group was 1.5-fold than that in control group,dermal AUC was 2.3-fold higher.The unit area fluorescent amount in both epidermis and dermis was highest 2 hours after topical medication in control group(1585.52/μm2 and 2005.66μm2),and quickly reduced after 4 hours.But the epidermal and dermal unit area fluorescent amount in LP group was rather low in 4 hours after topical medication,and gradually increased after6hours,and peaked after 12 hours(750.28/μm2 and 1073.08/μm2).Conclusion Liposome preparation of podophyllotoxin can be slowly released and lasts longer in the skin.

Key words: Liposomes, Podophyllotoxin, Administration,cutaneous