中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (8): 461-463.

• 论著 • 上一篇    下一篇

从野生蟾蜍分离致病性着色真菌的研究

万俊增, 戴文丽, 任忠芬, 陈瑞娥, 施岩   

  1. 山东省立医院皮肤性病科 济南 250021
  • 收稿日期:2003-01-06 出版日期:2003-08-15 发布日期:2003-08-15

Study on Isolation of Pathogenic Dematiaceous Fungi from Wild Toads

WAN Jun-zeng, DAI Wen-li, REN Zhong-fen, CHEN Rui-e, SHI Yan   

  1. Department of Dermatology, Shandong Provincial Hospital, Jinan 250021, China
  • Received:2003-01-06 Online:2003-08-15 Published:2003-08-15

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摘要： 目的 从野生蟾蜍分离致病性着色真菌。方法 在着色芽生菌病高发区和无病区分别捕捉野生蟾蜍,取内脏制成悬液分离着色真菌。结果 在着色芽生菌病高发区山东章丘,354只野生蟾蜍中有151只分离出着色真菌,占42.7%,共计分离出着色真菌11种,162株,其中疣状瓶霉49株,外瓶霉33株,瓶霉属19株,甄氏外瓶霉19株,卡氏枝孢12株,可疑卡氏枝孢3株,喙枝孢7株,裴氏着色霉5株,毛样枝孢4株,棘状外瓶霉3株,枝孢属3株,小尾枝孢1株,未鉴定4株。而在无病区山东莱阳,45只蟾蜍中仅有4只分离出非致病性着色真菌,占8.9%。结论 着色芽生菌病流行区野生蟾蜍真菌的分离阳性率显著高于非流行区,且分离出多种致病性着色真菌,提示野生蟾蜍带菌与着色芽生菌病的流行相关。

关键词: 有丝分裂孢子真菌, 蟾蜍科

Abstract: Objective To compare the carriage of dematiaceous fungi in wild toads from both endemic area and non-epidemic area of chromoblastomycosis.Methods A total of 354 and 45 wild toads(Bufo Chinese)were caught from two counties of Shandong Province,Zhangqiu county,an endemic area of chromoblastomycosis,and from Laiyang county,an area where the infection of dematiaceous fungi in humans has not been found,respectively.The toads were killed and the suspension of the internal organs were used to isolate dematiaceous fungi.Results Eleven species including 162 strains of dematiaceous fungi were isolated from 151 of 354(42.7%) toads from the endemic area.The isolated strains consisted of 49 Phialophora verrucosa,33 Exsophiala,19 Phialophora(nonpathogenic),19 E.jenselmei,12 Cladosporium carrionii,3 suspected of C.carrionii,7 Rhinocladiella sp.,5 Fonsecaea pedrosoi,4 C.trichoides,3 E.spinifera,1 C.minourae,4unidentified.Only 4 nonpathogenic dematiaceous fungi were isolated from 4 of 45(8.9%) toads obtained from non-epidemic area.Conclusions Varieties of pathogenic dematiaceous fungi,especially C.carrionii,are isolated from wild toads in endemic area of chromoblastomycosis,while none from non-endemic area.The results suggest that the colonization of pathogenic dematiaceous fungi in wild toads may be associated with endemicity of chromoblastomycosis.

Key words: Mitosoporic fungi, Bufonidae