中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (10): 572-573.

• 论著 • 上一篇    下一篇

体外培养正常人黑素细胞内皮素A受体mRNA表达的研究

杜娟, 徐前喜, 何培英, 闫征, 张建中, 朱铁君   

  1. 北京大学人民医院皮肤科 100044
  • 收稿日期:2002-11-05 出版日期:2003-10-15 发布日期:2003-10-15

Expression of ETA-R Gene in Cultured Normal Human Melanocytes

DU Juan, XU Qian-xi, HE Pei-ying, YAN Zheng, ZHANG Jian-zhong, ZHU Tie-jun   

  1. Department of Dermatology, People's Hospital, Peking University, Beijing 100044, China
  • Received:2002-11-05 Online:2003-10-15 Published:2003-10-15

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摘要： 目的 探讨正常人黑素细胞中是否存在内皮素-1的高选择性A受体(ETA-R)的mRNA表达。方法 采用体外黑素细胞培养技术和逆转录-聚合酶链反应(RT-PCR)技术检测正常人黑素细胞ETA-R的mRNA表达情况。结果 正常人黑素细胞有ETA-R的mRNA表达,凝胶电泳显示有特异性条带,以分子量标记电泳条带作为参照,cDNA产物片段长度约为400 bp。结论 内皮素-1可能是通过与其高选择性ETA-R结合而发挥其对黑素细胞的促增殖作用。

关键词: 黑素细胞, 内皮缩血管肽-1, 受体, 内皮缩血管肽

Abstract: Objective To explore the expression of high selective A receptor of endothelin-1 (ETA-R) in melanocytes from normal human beings. Methods Normal human melanocytes were cultured and mR-NA expression of ETA-R in melanocytes was measured by reverse transcription-polymerase chain reaction. Results The mRNA expression of ETA-R was seen in melanocytes. Conclusion This study combined with other experiments suggests that endothelin-1 may affect melanocytes by high selective ETA-R which may con-tribute to the pathogenesis of vitiligo.

Key words: Melanocytes, Endothelin-1, Receptors, endothelin