中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (12): 708-710.

• 论著 • 上一篇    下一篇

单纯疱疹病毒2型DNA疫苗对豚鼠抗病毒感染的研究

韩双艳1, 杨慧兰1, 王捷2, 冼江2, 杨太成2   

  1. 1. 广州军区广州总医院皮肤科 510010;
    2. 广州军区广州总医院医学实验科
  • 收稿日期:2003-02-10 出版日期:2003-12-15 发布日期:2003-12-15
  • 通讯作者: 杨慧兰,E-mail:huilany@hotmail.com E-mail:huilany@hotmail.com
  • 基金资助:
    国家自然科学基金(30271192);广东省科委重大攻关课题(010795)

Prophylactic Effects of a HSV-2 DNA Vaccine in Guinea Pigs

HAN Shuang-yan1, YANG Hui-lan1, WANG Jie2, XIAN Jiang2, YANG Tai-cheng2   

  1. Department of Dermatology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China
  • Received:2003-02-10 Online:2003-12-15 Published:2003-12-15

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摘要： 目的 探讨单纯疱疹病毒2型DNA疫苗的抗病毒感染效应.方法 重组DNA疫苗pcgD免疫雌性豚鼠,采用血清中和实验检测抗体,单纯疱疹病毒2型(HSV-2)sav株接种豚鼠,观察DNA疫苗保护动物抵抗HSV-2病毒感染的能力.结果 pc-gD免疫豚鼠产生的中和抗体效价远远高于对照组和生理盐水组,感染病毒后,表现出初发感染推迟,复发感染的严重程度下降.结论 DNA疫苗能有效地保护动物免受HSV-2病毒的攻击,延缓初发感染,减少复发感染.

关键词: 疫苗,DNA, 中和抗体, 疱疹病毒2型,人, 动物替代试验

Abstract: Objective To observe the prophylactic effects of a HSV-2 gD2 DNA vaccine in guinea pigs challenged with HSV-2 strains.Methods Female guinea pigs were divided into 3 groups with 10 each,which was immunized intramuscularly with 100μg of pc-gD plasmids(recombinant HSV-2 DNA vaccine),or with pc DNA 3 blank plasmids,with normal saline as control,respectively.Two booster injections were given on day 7 and day 21.Sera were collected for virus neutralization test on day 0,day 28,and day 56.The animals were challenged with HSV-2 strain sav intravaginally,and lesions induced on the external genital skin were scored between day1and day 21 after challenge.Results The titer of neutralizing antibody to HSV-2 was much higher in the sera from animals immunized by pc-gD plasmids than that from animals immunized by pc DNA 3 blank plasmids or normal saline.Furthermore,the lesion scores on external genital skin were significantly decreased in pc-gD group than those in other two groups with either primary or recurrent infections.Conclusion The constructed gD 2 vaccine can efficiently protect guinea pigs from genital infection and reduce recurrent infection induced by latent herpes simplex virus.

Key words: Vaccines,DNA, Herpesvirus2,human, Animal testing alternatives