中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (1): 41-43.

• 论著 • 上一篇    下一篇

白塞综合征患者抗血管内皮细胞抗体和抗心磷脂抗体的检测

蒋建华, 普雄明, 周炳华, 吴卫东, 刘玉琴   

  1. 新疆维吾尔自治区人民医院, 乌鲁木齐830001
  • 收稿日期:2003-05-26 出版日期:2004-01-15 发布日期:2004-01-15
  • 基金资助:

    杨森青年科研基金赞助

Detection of Antibodies Against Endothelial Cell Antigens and Anticardiolipin Antibodies in Patients with Behcet Disease

JIANG Jian-hua, PU Xiong-ming, ZHOU Bing-hua, WU Wei-dong, LIU Yu-qin   

  1. Department of Dermatology, the People's Hospital of Xin Jiang Uygur Autonomous Region, Urumqi 830001, China
  • Received:2003-05-26 Online:2004-01-15 Published:2004-01-15

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摘要：

目的 探讨血清抗血管内皮细胞抗体(AECA)及抗心磷脂抗体(ACA)在白塞综合征发病中的作用。方法 收集白塞综合征患者和正常人血清,用间接免疫荧光技术检测血清中AECA并分型,用酶联免疫吸附方法检测ACA。结果 白塞综合征组AECA阳性率为81.3%(32例中26例阳性),其中IgG-AECA阳性率为71.9%,IgM-AECA阳性率为53.1%,与正常人对照组比较差异有显著性(P<0.01)。同时AECA阳性组与阴性组比较在发生结节性红斑或结节性血管炎及血沉增快方面前者高于后者。ACA检测结果,白塞综合征组阳性率为53.1%,显著高于正常人对照组。ACA阳性组与阴性组比较,在口腔溃疡、皮肤、外生殖器及贫血等临床表现及血沉等方面差异无显著性(P>0.05)。结论 白塞综合征患者血清AECA水平对白塞综合征血管损害及病情活动具有一定意义。ACA对病情的影响尚需进一步研究。

关键词: 贝赫切特综合征, 免疫球蛋白类, 抗体,抗磷脂

Abstract:

Objective To study the correlation between serum level of antiendothelial cells auto-antibodies (AECA) and anticardiolipin antibodies (ACA) and the roles in the pathogenesis of Behcet's disease (BD). Methods Thirty two BD patients were randomly selected from our outpatients and inpatients. Thirty-three healthy controls were studied in parallel. AECA/ACA were examined by indirect immunoflourescence technique and enzyme linked immunosorbent assay (ELISA) respectively, IgG-AECA and IgM-AECA antibodies were detected, too. Results The positivity rates of AECA, IgG-AECA and IgM-AECA in BD patients were 81.3%, 71.9% and 53.1%, respectively. Increased serum AECA levels were observed in all BD patients compared with those of normal controls. There was some significant positive correlation in the positivity rate of AECA between erythema nodosum, nodular vasculitis and rapid erythrocyte sedimentation rate. The positivity rate of ACA was 53.1%, A significant difference in the positivity rate of ACA compared with those of normal controls. Neither of these two kinds antibodies was significantly correlated with oral ulcers, clinical manifestations of skin and external genital organ, anemia or erythrocyte sedimentation rate. Conclusion The detection of AECA level is useful to identify cutaneous vasculitis and the activity of disease in BD, but the value of ACA in the diagnosis of the condition of BD needs further study.

Key words: Behcet’s syndrome, Immunoglobulins, Antibodies, antiphospholipid