中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (2): 94-96.

• 论著 • 上一篇    下一篇

发夹状RNA对人乳头瘤病毒16型E6表达的影响

钟绮丽1, 李建军1, 程滨珠1, 于洁2   

  1. 1. 北京大学深圳医院皮肤性病科 深圳 518036;
    2. 北京大学深圳医院中心实验室 深圳 518036
  • 收稿日期:2003-09-15 出版日期:2004-02-15 发布日期:2004-02-15

The Effects of Hairpin RNA on the Expression of Human Papillomavirus E6 Gene

ZHONG Qi-li1, LI Jian-jun1, CHENG Bin-zhu1, YU Jie2   

  1. Department of Dermatology, Shenzhen Hospital, Beijing University, Shenzhen 518036, China
  • Received:2003-09-15 Online:2004-02-15 Published:2004-02-15

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摘要： 目的 研究发夹状RNA对人乳头瘤病毒(HPV)E6基因表达的影响。方法 构建发夹状RNA、正义RNA及反义RNA表达质粒,重组体转入人宫颈癌细胞株CaSKi,应用RT-PCR及蛋白印迹法检测其对CaSKi细胞E6mRNA及蛋白表达的影响。结果 发夹状RNA表达质粒的导入明显下调了E6mRNA及蛋白表达,发现含有发夹状RNA的HPV-E6mRNA表达水平相当于空白对照组的14.6%,蛋白表达水平相当于空白对照组的23.3%,此效果优于传统的反义RNA。结论 利用发夹状RNA的干扰技术可有效抑制靶基因的表达。

关键词: 乳头状瘤病毒,人, RNA,信使, 质粒, 基因表达

Abstract: Objective To investigate the effects of hairpin RNA on the expression of human papillomavirus E6 gene. Methods Plasmids expressing hairpin RNA, sense RNA and anti-sense RNA were constructed. The recombinants were transfected into cervical cancer cell line, CaSKi. Expression of E6 was detected with semi-quantitative RT-PCR and Western blot. Results Hairpin RNA could significantly reduce the expressions of E6 mRNA and protein, which was more efficient than traditional anti-sense RNA did. Conclusion The RNA interference through hairpin RNA can effectively inhibit the expression of target gene, and can be potentially useful in gene therapy of HPV related tumors.

Key words: Papillomavirus, human, RNA, messenger, Plasmids, Gene expression