中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (2): 74-76.

• 论著 • 上一篇    下一篇

副肿瘤性天疱疮伴发Castleman肿瘤分泌致病相关抗体的初步研究

王亮春, 陈喜雪, 赵俊郁, 卜定方, 朱学骏   

  1. 北京大学第一医院皮肤科 100034
  • 收稿日期:2003-05-08 出版日期:2004-02-15 发布日期:2004-02-15
  • 基金资助:

    国家自然科学基金资助(30371292)

A Preliminary Study on the Antibody Secreted in Castleman’s Disease Associated with Paraneoplastic Pemphigus

WANG Liang-chun, CHEN Xi-xue, ZHAO Jun-yu, BU Ding-fang, ZHU Xue-jun   

  1. Department of Dermatology, Peking University First Hospital, Beijing 100034, China
  • Received:2003-05-08 Online:2004-02-15 Published:2004-02-15

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摘要：

目的 Castleman肿瘤组织细胞培养及培养细胞上清液中抗体成分性质的鉴定。方法 分离副肿瘤性天疱疮患者的肿瘤淋巴细胞进行培养,将培养细胞上清液超滤离心获得浓缩的IgG抗体为一抗,分别以鼠膀胱冰冻切片为底物行间接免疫荧光检查,以正常皮肤蛋白提取物为底物进行免疫印迹试验。RT-PCR,克隆测序分析培养肿瘤B细胞免疫球蛋白重链基因并与其他6例该病患者的基因相比较。结果 培养细胞上清液中的IgG抗体能够在鼠膀胱上皮细胞间沉积;并且这些抗体能够识别皮肤中分子量分别为210000和190000的两种抗原成分。扩增培养肿瘤B细胞免疫球蛋白重链基因得到单一锐利条带,克隆测序发现该基因与另外6例患者的基因序列具有高度一致性。结论 患者肿瘤组织能够分泌抗体,并且分泌抗体的性质与血液中抗体性质基本相同。

关键词: 副肿瘤性天疱疮, 序列分析

Abstract:

Objective To identify features of antibodies in the supernatants of cultured Castleman's disease cells.Methods Lymphocytes of Castleman's disease were isolated and cultured.Immunofluorescence and immunoblot assays were performed with IgG extracted from culture supernatants.The immunoglobulin heavy chaingene of cultured tumor B cells was analyzed by RT-PCR,cloning and sequencing.Results Ig Gextracted from culture supernatant scould attach to the epithelial cell surfaces of mouse bladder tissues.The antibody could also identify two antigen components,210000 and 190000,of normal human epidermal tissues.The sequence similarity was found in immunoglobulin heavy chain gene of cultured tumor Bcells com-pared with that of 6 patients with Castleman's disease previously reported. Conclusions Castleman's tumor associated with paraneoplastic pemphigus can secret autoantibody with similar features to that found in patients'sera.

Key words: Paraneoplastic pemphigus, Sequence analysis