中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (4): 209-211.

• 论著 • 上一篇    下一篇

两种小鼠皮肤硬化模型的对比研究

赵凯, 屠文震   

  1. 上海市中西医结合医院硬皮病科
  • 收稿日期:2003-04-11 出版日期:2004-04-15 发布日期:2004-04-15

Comparative Study on Two Mouse Models of Sclerotic Skin

ZHAO Kai, TU Wen-zhen   

  1. Scleroderma Ward, Integrated Traditional Chinese Medicine with Western Medicine Hospital, Shanghai 200082, China
  • Received:2003-04-11 Online:2004-04-15 Published:2004-04-15

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摘要： 目的 建立小鼠皮肤硬化模型,并比较不同品系的C3H和BALB/c小鼠对博来霉素诱导皮肤硬化的敏感性.方法 采用以磷酸盐缓冲液(PBS)稀释的质量浓度为200μg/mL的博来霉素0.1mL于小鼠背部皮下注射3周,对照组以0.1mLPBS于小鼠背部皮下注射3周.注射结束后,对小鼠皮肤、肺组织的病理改变、皮肤厚度的改变和胶原含量等方面进行对比.结果 C3H和BALB/c小鼠在博来霉素诱导下均发生皮肤硬化,硬化皮肤厚度及皮肤胶原含量(6mm×6mm)均显著高于对照组(P<0.01);通过比较发现C3H小鼠在皮肤厚度改变、胶原含量改变等方面均明显高于BALB/c小鼠(P<0.05),而产生皮肤硬化的时间短于BALB/c小鼠.结论 应用博来霉素可在短期内诱导出C3H和BALB/c小鼠皮肤硬化,成功建立小鼠皮肤硬化模型.二者比较C3H小鼠对博来霉素较敏感,为博来霉素诱导硬皮病样模型的理想动物.

关键词: 硬皮病,局限性, 博来霉素, 小鼠,近交C3H, 小鼠,近交BALBc, 模型,动物

Abstract: Objective To establish the mouse model of sclerotic skin. Methods The sclerotic skin was induced by local injections of bleomycin (BLM) in C3H and BALB/c mice. The injection solution was prepared with BLM at the concentration of 200μg/mL in PBS. In the test group 0.1 mL BLM solution was injected daily into the back of the mice for 3 weeks. For the control mice same amount of PBS was injected daily for 3 weeks. After 3 weeks, the histology of the skin and lungs was compared between the different groups, also the changes of skin thickness and quantity of collagen. Results After the treatment of BLM sclerotic skin was observed in both C3H and BALB/c mice, in which the thickness of skin and the quantity of collagen (6 mm×6 mm) were higher than those in the control mice (P<0.01). The study showed that in C3H mice the BLM-induced skin changes were more prominent than those in BALB/c mice (P<0.05) and the induction period of sclerotic skin was shorter. Conclusions The mouse model of sclerotic skin could be established by injection of BLM within several weeks. The C3H mouse is more sensitive to the induction by BLM, which indicates that C3H mouse may be a better animal model for the induction of sclerotic skin.

Key words: Scleroderma, circumscribed, Bleomycin, Mice, inbred C3H, Mice, inbred BALB c, Models, animal