中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (6): 347-350.

• 论著 • 上一篇    下一篇

全反式维A酸对内皮细胞迁移能力及明胶酶表达的影响

邓辉, 阎春林, 严淑贤, 胡跃, 徐昱, 廖康煌   

  1. 复旦大学附属华山医院皮肤科 上海 200040
  • 收稿日期:2003-06-29 出版日期:2004-06-15 发布日期:2004-06-15
  • 通讯作者: 阎春林,E-mail:yancl@sh.cnuninet.net E-mail:yancl@sh.cnuninet.net

All-Trans-Retinoid Effecting Endothelial Cell Migration and Gelatinase Expression

DENG Hui, YAN Chun-lin, YAN Shu-xian, HU Yue, XU Yu, LIAO Kang-huang   

  1. Department of Dermatology, Huashan Hospital of Fudan University, Shanghai 200040, China
  • Received:2003-06-29 Online:2004-06-15 Published:2004-06-15

PDF (PC)

109

赞

摘要： 目的 研究全反式维A酸(All-trans retinoid,ATRA)对内皮细胞迁移能力及明胶酶表达的影响.方法 应用迁移实验研究了全反式维A酸对内皮细胞迁移能力的影响,应用半定量逆转录-聚合酶链反应及蛋白印迹检测了全反式维A酸对明胶酶表达的影响,同时应用明胶酶谱法观测了全反式维A酸对明胶酶活性的影响.结果 0.1μmol/L、1.0μmol/L、10.0μmol/L浓度的全反式维A酸可明显抑制佛波酯(PMA)诱导的内皮细胞迁移,抑制率分别为(44.68±7.79)%、(65.20±4.59)%及(78.37±2.58)%.同时全反式维A酸可影响明胶酶mRNA的转录进而下调其蛋白表达量及酶活性,此作用随浓度增高而增强,10.0μmol/L浓度的全反式维A酸对明胶酶AmRNA、蛋白表达量及酶活性的抑制率分别为(59.39±7.98)%、(78.40±3.23)%及(53.02±7.23)%,对明胶酶BmRNA、蛋白表达量及酶活性的抑制率分别为(65.23±3.62)%、(82.49±2.88)%及(47.32±7.72)%.结论 全反式维A酸可在体外抑制佛波酯诱导的内皮细胞迁移,此作用可能是通过其对明胶酶表达及活性的抑制来实现.

关键词: 维甲酸, 内皮, 明胶酶A, 明胶酶B, 细胞,培养的

Abstract: Objective To investigate the effect of all-trans-retinoid(ATRA) on the migration of endothelial cells and the expression of gelatinases. Methods In vitro migration assay was used to determine the effect of ATRA on the endothelial cell migration induced by phorbol 12-myristate-13-acetate (PMA), semi-quantitative RT-PCR and Western blot were used to observe the effect of ATRA on the gelatinase expression at mRNA and protein levels respectively, while the proteolytic activities of gelatinases were assessed by zymography. Results The endothelial cell migration elicited by PMA was significantly inhibited when incubated with 0.1 μmol/L, 1.0 μmol/L and 10.0 μmol/L ATRA. Compared with the control, the inhibition rates were (44.68±7.79)%, (65.20±4.59)% and (78.37±2.58)%, respectively. ATRA also reduced the expression and activities of gelationases in a dose dependant manner. At 10 μmol/L concentration, the inhibition rate of mRNA expression, protein expression and protein activities for gelatinase A was (59.39±7.98)%, (78.40±3.23)% and (53.02±7.23)%, respectively. For gelatinase B it was (65.23±3.62)%, (82.49±2.88)% and (47.32±7.72)%, respectively. Conclusions The expression and activities of gelatinases are downregulated in the endothelial cells when incubated with ATRA, which may be the possible mechanism of ATRA inhibiting the endothelial cell migration elicited by PMA in vitro.

Key words: Tretinoin, Endothelium, Gelatinase A, Gelatinase B, Cell, cultured