中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (8): 484-486.

• 论著 • 上一篇    下一篇

疑似深部真菌感染患者血中(1→3)β-D-葡聚糖的检测

席丽艳, 鲁长明, 李军, 谢穗生   

  1. 中山大学附属第二医院皮肤科 广州 510120
  • 收稿日期:2003-08-14 出版日期:2004-08-15 发布日期:2004-08-15
  • 基金资助:
    国家自然科学基金资助(39970043)

Assay of Plasma (1→3)-β-D-glucan in Patients Suspected with Deep Fungal Infection

XI Li-yan, LU Chang-ming, LI Jun, XIE Sui-sheng   

  1. Department of Dermatology, The Second Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510120, China
  • Received:2003-08-14 Online:2004-08-15 Published:2004-08-15

PDF (PC)

87

赞

摘要： 目的 探讨疑似深部真菌感染患者血中(1→3)-β-D-葡聚糖的浓度与感染的关系。方法 采用日本生化学工业株式会社的(1→3)-β-D-葡聚糖测定G-testTE试剂盒检测了13例患者血浆中的(1→3)-β-D-葡聚糖的含量,以UV-2450紫外可见光分光光度仪检测波长545nm的吸光度值,根据特定检测浓度换算公式得出标本的(1→3)-β-D-葡聚糖含量。结果 13例被检测患者中9例经培养证实为深部真菌感染者血浆的β-D-葡聚糖含量皆较高,最高者可达352.94pg/mL(为真菌混合感染患者),平均可达203.47pg/mL;4例真菌培养阴性患者血浆β-D-葡聚糖水平,3例超过54.40pg/mL,1例为16.16pg/mL。所有标本采用UV-2450紫外可见光分光光度仪在波长545nm检测,皆可出现较明显的吸收峰。采用GtestTE试剂盒检测13例疑似深部真菌感染患者血浆的β-D-葡聚糖含量,分析诊断深部真菌感染的有效性,结果其敏感度为92.31%,特异度100%,阳性预测值100%,阴性预测值为98.36%。结论 GtestTE方法简便,反应快速,可用于真菌感染的早期诊断。

关键词: 真菌病, 葡聚糖类

Abstract: Objective To investigate the relationship between the concentration of plasma (1→3)-β-D-glucan (β-D-glucan) and deep fungal infection. Methods Thirteen patients were recruited in this study, who were suspected with deep fungal infection. G-test TE reagent for β-D-glucan measurement was used to detect the plasma (1→3) β-D-glucan in the patients by using UV-2450 spectrophotometer at 545 nm wavelength. The final concentrations were calculated according to concentration conversion formula. Results Nine of thirteen patients were confirmed as deep fungal infection by positive tissue culture, in whom high concentrations of β-D-glucan were detected, the highest concentration was 352.94 pg/mL (mixed infection), with a mean value of 203.47 pg/mL. In the other four patients with negative culture, the β-D-glucan concentration was over 54.40 pg/mL in three patients and 16.16 pg/mL in the another. Our results showed that the sensitivity, specificity, positive predictive value and negative predictive value of this test were 92.31%, 100%, 100% and 98.36%, respectively. Conclusion G-test TE method is a simple and rapid test and may be used for the diagnosis of patients with deep fungal infection.

Key words: Mycoses, Glucans