中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (9): 525-527.

• 论著 • 上一篇    下一篇

自体疣植入治疗扁平疣及其相关机制研究

曹碧兰1, 郭瑞珍2, 肖庆邦2, 陈晓红1, 晏文1   

  1. 1. 贵州省遵义医学院附属医院皮肤科, 563003;
    2. 贵州省遵义医学院附属医院病理科, 563003
  • 收稿日期:2003-09-24 出版日期:2004-09-15 发布日期:2004-09-15

Clinical and Experimental Study on Autoimplantation Technique in the Treatment of Verruca Plana

CAO Bi-lan1, GUO Rui-zhen2, XIAO Qing-bang2, CHEN Xiao-hong1, YAN Wen1   

  1. Department of Dermatology, The Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China
  • Received:2003-09-24 Online:2004-09-15 Published:2004-09-15

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摘要： 目的 应用自体疣植入治疗扁平疣,研究患者T细胞亚群和白介素2(IL-2)水平的变化以及疣体脱落的病理和免疫学特征。方法 采用单克隆抗体碱性磷酸酶-抗碱性磷酸酶(APAAP)法和放射免疫法检测患者外周血T细胞亚群及IL-2水平;对反应期疣体组织进行常规HE染色和免疫组织化学分析。结果 治疗前外周血CD4+细胞数、CD4+/CD8+比值及IL-2水平均明显低于正常人对照组(P<0.01);治疗后均显著升高(P<0.01),与正常人对照组差异无显著性(P>0.05)。反应期疣体组织中见淋巴细胞浸润,伴棘细胞水肿、溶解。免疫组化显示,反应区浸润的淋巴细胞主要呈CD45RO+、CD6+、CD4+、CD8+、CD20-和Mac387-;在表皮基底层及反应区可见S-100蛋白阳性的朗格汉斯细胞。结论 自体疣植入治疗扁平疣能增强细胞免疫功能,通过T细胞介导免疫反应使CD8+亚群发挥细胞毒性作用,导致疣体脱落。

关键词: 疣, 埋藏疗法, T淋巴细胞, 白细胞介素2, 病理学,临床

Abstract: Objective To apply autoimplantation technique in the treatment of verruca plana, and to investigate the mechanisms of it. Methods The changes of T cell subsets and IL-2 level in the peripheral blood of the patients with verruca plana, in conjunction with histopathological and immunohistochemical features of verruca plana tissue at reactive stage were studied by APAAP and radioimmunoassay, respectively. The tissue specimens were studied by H.E stain and immunohistochemical techniques. Results Significantly decreased CD4+ cell numbers, CD4+/CD8+ ratio and IL-2 level were found before treatment, compared with those in healthy controls (P<0.01). However, these indicators were shown significantly increased after treatment, compared with those before treatment (P<0.01), and there were no significant differences from the controls (P>0.05). Lymphocytic infiltration, epidermal edema and dissolution were shown in the histopathological study. There were CD45RO+, CD6+, CD4+ and CD8+ cells in the lymphocytic infiltration, but no CD20+ and Mac387- cells. Langerhans cells with positive S-100 protein were observed in basal layer of the epidermis and reaction area. Conclusions T cell-mediated immune responses could be improved in autoimplantation, the warts are rejected mainly by of CD8+ subset cytotoxicity.

Key words: Warts, Embedding therapy, T-lymphocytes, Interleukin-2, Pathology, clinical