中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (9): 512-514.

• 论著 • 上一篇    下一篇

维生素D3及其类似物对树突细胞表达和分泌白介素10的作用

朱可建, 劳力民, 茅晓红   

  1. 浙江大学医学院附属第二医院皮肤科, 杭州310009
  • 收稿日期:2003-09-13 出版日期:2004-09-15 发布日期:2004-09-15
  • 通讯作者: 朱可建,E-mail:zhukdejian@mail.hz.zj.cn E-mail:zhukdejian@mail.hz.zj.cn
  • 基金资助:
    国家教委留学回国人员基金资助项目;浙江省卫生厅科学研究基金(2000A119)

1, 25-Dihydroxyvitamin D3 and Its Analogues Modulate the Expression and Secretion of IL-10 by Human Monocyte-derived Dendritic Cells

ZHU Ke-jian, LAO Li-min, MAO Xiao-hong   

  1. Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
  • Received:2003-09-13 Online:2004-09-15 Published:2004-09-15

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摘要： 目的 研究1,25-二羟维生素D3及其类似物卡泊三醇和他卡西醇对来源于单核细胞的树突细胞(DC)表达和产生白介素10(IL-10)的调节作用。方法 在体外将人外周血单核细胞分化成树突细胞。分别采用半定量RT-PCR和ELISA法分析1,25-二羟维生素D3及其类似物对MDDC表达IL-10mRNA和产生IL-10蛋白的影响。并以1,25-二羟维生素D3的代谢物24,25-二羟维生素D3作对照。结果 1,25-二羟维生素D3、卡泊三醇和他卡西醇以相当的作用强度上调MDDC表达和产生IL-10,但24,25-二羟维生素D3未显示调节作用;1,25-二羟维生素D3对IL-10蛋白水平的上调作用呈剂量依赖方式。结论 1,25-二羟维生素D3及其类似物治疗银屑病的作用可能是通过调节IL-10的表达和分泌来实现的。

关键词: 1,25-二羟维生素D3, 树突状细胞, 白介素-10

Abstract: Objective To elucidate the therapeutic mechanism of 1,25-dihydroxyvitamin D3 and its analogues in psoriasis. Methods The modulating role of calcitriol, calcipotriol and tacalcitol on the expression and secretion of IL-10 in monocyte-derived dendritic cells (MDDCs) was investigated. Human monocytes were differentiated into MDDCs in vitro in the presence of GM-CSF and IL-4. Semi-quantitative RT-PCR and ELISA were used to analyze the mRNA expression and protein production of IL-10 in MDDCs. In each experiment, 24,25-dihydroxyvitamin D3 (a vitamin D3 metabolite with a low affinity to the vitamin D receptor) was used as a control. Results The mRNA expression and protein secretion of IL-10 in MDDCs was up-regulated by calcitriol, calcipotriol or tacalcitol with a similar potency, but not by 24,25-dihydroxyvitamin D3. The up-regulatory role of calcitriol on IL-10 protein secretion was dose dependent. Conclusion The therapeutic effect of calcitriol and its analogues in the treatment of psoriasis may be related to the induction of IL-10 production.

Key words: 1, 25-Dihydroxyvitamin D3, Dendritic cells, Interleukin-10