中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (10): 575-577.

• 论著 • 上一篇    下一篇

恶性黑素瘤内皮型一氧化氮合酶和血管内皮生长因子的表达

陶娟, 涂亚庭   

  1. 华中科技大学同济医学院附属协和医院皮肤科 武汉 430022
  • 收稿日期:2003-10-18 出版日期:2004-10-15 发布日期:2004-10-15

Expression of Endothelial Nitric Oxide Synthase and Vascular Endothelial Growth Factor in Human Malignant Melanoma

TAO Juan, TU Ya-ting   

  1. Department of Dermatology, Affiliated Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430022, China
  • Received:2003-10-18 Online:2004-10-15 Published:2004-10-15

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摘要： 目的 探讨内皮型一氧化氮合酶(eNOS)和血管内皮生长因子(VEGF)与人恶性黑素瘤血管生成的关系。方法 免疫组化法检测31例人恶性黑素瘤标本eNOS、VEGF和第Ⅷ因子相关抗原(FⅧRAg)血管内皮细胞特异性染色,计数肿瘤微血管密度(MVD)。结果 24例恶性黑素瘤组织表达eNOS,26例表达VEGF,色素痣组织不表达eNOS和VEGF;VEGF与eNOS的表达呈正相关;eNOS、VEGF的表达与恶性黑素瘤MVD呈正相关,恶性黑素瘤MVD明显高于色素痣;eNOS、VEGF表达与淋巴结转移无关。结论 MVD随着eNOS和VEGF表达的增强而增加。

关键词: 黑色素瘤, 一氧化氮合酶, 内皮生长因子, 新生血管化, 病理性

Abstract: Objective To investigate the relationship between the expression of endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF) and the angiogenesis in human malignant melanoma (MM). Methods Tissue sections from 31 MM patients were examined. The protein expression of eNOS and VEGF was detected using immunohistochemistry technique and morphological quantitative analysis. Microvessel density (MVD) was also counted by immunostaining with anti-FⅧRAg antibody. Results (1)The positive staining of eNOS and VEGF was detected in 71.4% (24 cases) and 83.9% (26 cases) of MM respectively. As negative control there was no staining in the nevus pigmentosus tissue sections. (2)There was a positive correlation between the expression of eNOS and VEGF in MM. (3)The positive correlation was also found between the MVD and the expression of eNOS and VEGF in MM. The number of MVD was higher in MM than that in nevus pigmentosus tissue. (4)The lymphatic metastasis was not correlated with the expression of eNOS and VEGF. Conclusion The number of the MVD increases along with the high expression of eNOS and VEGF in MM.

Key words: Melanoma, Nitric-oxide synthase, Endothelial growth factors, Neovascularization, pathologic