中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (10): 569-571.

• 论著 • 上一篇    下一篇

尖锐湿疣患者角质形成细胞中活化型细胞外信号调控蛋白激酶和活化型p38的检测

苏明1, 程浩3, 盛彩霞4, 吴能定2, 李凌1, 项力俭1, 李雅芬1, 钟剑波1   

  1. 1. 杭州市第一人民医院皮肤科 310006;
    2. 杭州市第一人民医院皮肤科 310006;
    3. 浙江大学邵逸夫医院皮肤科;
    4. 浙江大学邵逸夫医院病理科
  • 收稿日期:2003-10-07 出版日期:2004-10-15 发布日期:2004-10-15
  • 基金资助:
    杭州市医药卫生青年研究基金(0203)

Expression of Activated ERK and p38 Kinase in HPV-Infected Keratinocytes

SU Ming1, CHENG Hao3, SHENG Cai-xia4, WU Neng-ding2, LI Ling1, XIANG Li-jian1, LI Ya-fen1, ZHONG Jian-bo1   

  1. Department of Dermatology, The First Hospital of Hangzhou, Hangzhou 310006, China
  • Received:2003-10-07 Online:2004-10-15 Published:2004-10-15

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摘要： 目的 探讨尖锐湿疣皮损角质形成细胞中活化型细胞外信号调控蛋白激酶(p-ERK)和活化型p38(p-p38)的表达。方法 采用原位杂交方法诊断HPV6/11相关尖锐湿疣50例;采用免疫组化EnVision二步法检测50例尖锐湿疣皮损中p-ERK和p-p38的表达及分布,并以25例正常人皮肤(包皮)作为对照。结果 ①p-ERK和p-p38在尖锐湿疣角质形成细胞中的表达较正常人上皮中均有不同程度的增强,前者u=4.113,P<0.01;后者u=4.497,P<0.01,其表达主要分布于棘细胞层,阳性信号均定位于胞核。②尖锐湿疣皮损中,p-ERK与p-p38的表达呈显著正相关(r=0.42,P<0.01)。结论 HPV6/11阳性尖锐湿疣患者中,p-ERK和p-p38的表达较正常人皮肤明显增强,提示在HPV6/11感染时,角质形成细胞中激酶ERK和p38的激活增加,可能通过ERK和p38MAPK通路起作用。

关键词: 尖锐湿疣, 乳头状瘤病毒, 人, 有丝分裂素激活蛋白激酶激酶类, p38

Abstract: Objective To investigate the expression of activated ERK (p-ERK) and activated p38 (p-p38) in the keratinocytes of condyloma acuminata (CA) lesions. Methods Fifty cases of HPV 6/11 CA were diagnosed by in situ hybridization. The expression and distribution of p-ERK and p-p38 in CA lesions and 25 normal human skins (foreskins) were detected by immunohistochemistry technique (En Vision). Results ①The results showed that the expression of p-ERK and p-p38 in keratinocytes of CA lesions were significantly higher than those in normal epidermis (P<0.01), and the expression distributed mainly in prickle layer of CA lesions. The positive signal located mainly in the nuclei. ②In CA lesions the expression of p-ERK and p-p38 was positively correlated. Conclusions The study suggests that, for CA caused by HPV 6/11, both ERK and p38 MAPK may play a role in MAP kinase signaling pathway.

Key words: Condylomata acuminata, Papillomavirus, human, Mitogen-activated protein kinase kinases, p38