中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (12): 698-700.

• 论著 • 上一篇    下一篇

外阴鳞状细胞癌中人乳头状瘤病毒感染与基质金属蛋白酶9和金属蛋白酶抑制剂1的关系

姚济芬1, 郑伟2, 王水英1, 付晓敏1, 石一复2   

  1. 1. 杭州市第二人民医院 310015;
    2. 浙江大学医学院附属妇产科医院
  • 收稿日期:2003-12-28 出版日期:2004-12-15 发布日期:2004-12-15

HPV Infection and its Correlation with MMP-9 and TIMP-1 Expression in Vulvar Squamous Cell Carcinoma

YAO Ji-fen1, ZHENG Wei2, WANG Shui-ying1, FU Xiao-min1, SHI Yi-fu2   

  1. The Second People's Hospital of Hangzhou, Hangzhou 310015, China
  • Received:2003-12-28 Online:2004-12-15 Published:2004-12-15

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摘要： 目的 探讨人乳头状瘤病毒(HPV)6/11、16/18在外阴鳞状细胞癌(简称外阴鳞癌)组织中的感染情况及与基质金属蛋白酶9(MMP-9)和金属蛋白酶抑制剂1(TIMP-1)蛋白表达的关系.方法 用原位杂交法检测HPV6/11、16/18在26例外阴鳞癌、21例外阴上皮内瘤病变(VIN)及10例外阴正常皮肤组织中的感染情况.同时用免疫组化法检测MMP-9、TIMP-1蛋白的表达.结果 HPV6/11、16/18在外阴鳞癌、VIN中的感染率分别为69.23%(18/26)和38.46%(10/26),42.86%(9/21)和28.57%(6/21),正常对照组没有感染.MMP-9、TIMP-1蛋白在外阴鳞癌、VIN、正常对照组中的阳性表达率分别为92.31%(24/26)和76.92%(20/26),90.48%(19/21)和80.95%(17/21),80.00%(8/10)和50.00%(5/10).HPV6/11的外阴鳞癌组、VIN组与正常对照组比较差异有显著性(P<0.05),HPV16/18的外阴鳞癌组与正常对照组比较差异有显著性.外阴病变各组MMP-9、TIMP-1阳性表达率与正常对照组比较差异均有显著性.在外阴鳞癌中MMP-9蛋白表达较TIMP-1蛋白表达强(P<0.05).MMP-9在有HPV6/11和HPV16/18感染患者中的表达较无感染者强,而TIMP-1蛋白的表达差异无显著性.结论 在外阴鳞癌的发生发展中HPV的感染起一定作用,当MMP-9的表达强于TIMP-1表达时可能是外阴鳞癌侵袭的一个指标,HPV感染可能会进

关键词: 癌,鳞状细胞, 乳头状瘤病毒,人, 明胶酶B, 金属蛋白酶1组织抑制剂

Abstract: Objectives To investigate HPV infection and its correlation with the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibito r of metalloproteinase-1 (TIMP-1) in vulvar squamous cell carcinoma (VSCC).Methods HPV6/11 and 16/18 DNA were detected using in situ hybridization in 26 cases of VSCC, 21 cases of vulvar intraepithelial neoplasia (VIN) and 10 specimens of normal skin.The expression of MMP-9 and TIMP-1 was measured by immunohistochemical method in these three groups.Results The infection rates of HPV6/11 and 16/18 in VSCC and VIN were 69.23% (18/26) and 38.46% (10/26), 42.86% (9/21) and 28.57% (6/21) respectively, and no HPV6/11 or 16/18 DNA was discovered in normal skin epidermis.The expression rates of MMP-9 and TIMP-1 in VSCC, VIN and normal skin epidermis were 92.31%(24/26) and 76.92% (20/26), 90.45% (19/21) and 80.95% (17/21), and 80.00% (8/10) and 50.00% (5/10) respectively.MMP-9 expression in HPV positive lesions was higher than that in HPV negative lesions, but no significant difference of TIMP-1 expression was observed in HPV positive and negative lesions.Conclusions HPV infection may play a role in the development of VSCC.The higher expression of MMP-9 comparing with that of TIMP-1 may be an early marker of tumor progression in VSCC, and HPV infection may facilitate the invas ion and metastasis of VSCC.

Key words: Carcinoma,squamous cell, Papillomavirus,human, Gelatinase B, Tissue-inhibitor of metalloproteinase-1