中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (12): 687-689.

• 论著 •    下一篇

CpG序列在诱生抗dsDNA抗体中的作用

李智铭1, 徐世正2, 林孝华1, 林霖霖1, 章泳2, 李秉煦1   

  1. 1. 浙江温州医学院第一附属医院皮肤科 325000;
    2. 武汉大学人民医院皮肤科
  • 收稿日期:2003-12-28 出版日期:2004-12-15 发布日期:2004-12-15

The Role of CpG Sequences in the Induction of Anti-dsDNA Antibodies

LI Zhi-ming1, XU Shi-zheng2, LIN Xiao-hua1, LIN Lin-lin1, ZHANG Yong2, LI Bing-xu1   

  1. Department of Dermatology, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China
  • Received:2003-12-28 Online:2004-12-15 Published:2004-12-15

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摘要： 目的 研究CpG序列在诱生抗dsDNA抗体中的作用及可能机制.方法 以人工合成的含有CpG序列的寡核苷酸为生物佐剂,用天然的小牛胸腺DNA模拟自身抗原,免疫正常的BALB/c小鼠.免疫结束后,分别用天然的大肠杆菌DNA(nECDNA)和小牛胸腺DNA(nCTDNA)作为包被抗原检测小鼠血清的抗dsDNA抗体.结果 用CpG序列加nCTDNA免疫的小鼠出现较高水平的抗dsDNA抗体和细胞因子(包括白介素6、白介素12、干扰素γ),组间的差异有显著性(P<0.01),诱导的抗体与nECDNA和nCTD-NA均能结合,但在结合的量上有差异.结论 CpG序列能促进正常小鼠产生具有交叉反应特性的抗ds-DNA抗体.

关键词: 红斑狼疮,系统性, 抗体,抗核, 寡核苷酸序列分析

Abstract: Objective To investigate the effect of CpG sequences on the induction of anti-dsDNA antibodies and the possible mechanism.Methods Using a synthetic oligodeoxynucleotide (CpG-ODN) containing CpG as an adjuvant, native calf thymus DNA (nCTDNA) was used as the mimic self antigen to immunize normal BALB/c mice.About one week after immunization, anti-dsDNA antibodies were tes ted by ELISA using nCTDNA and native Escherichia coli DNA (nECDNA) as test antigens.Results The levels of anti-dsDNA antibodies and cytokines including IL-6, IL-12, IFN-γ in the sera from the mice immunized with CpGODN plus nCTDNA were signigicantly higher than those of othet immunization groups (P<0.01).These induced antibodied could bind both nCTDNA and nECDNA, but the binding abilities were different significantly.Conclusion The CpG motif can promote the production of cross-reactive anti-dsDNA antibodies in normal mice.

Key words: Lupus erythematosus,systemic, Antibodies,antinuclear, Oligonucleotide array sequence analysis