中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (11): 767-770.

• 论著 •    下一篇

178例儿童脓疱疮皮损中金黄色葡萄球菌药敏及随机扩增多态性DNA分析

卞彩云1,路永红2,周培媚2,王敏3,陈明1,蒋存火1,蔡琦2,李煊2,张大维1,冉琴3,徐海涛3,陈前明2,刘艳2,吴波1,王振远2,陈娜伊2,向丹黎2,黄旭蕾4,保勇1,吕燕5,尹亚菲5   

  1. 1. 成都市第二人民医院
    2. 成都市第二人民医院皮肤科
    3. 成都市儿童医院
    4. 四川大学华西医院
    5. 成都市第二人民医院检验科
  • 收稿日期:2012-01-06 修回日期:2012-03-02 出版日期:2012-11-15 发布日期:2012-10-31
  • 通讯作者: 卞彩云 E-mail:biancaiyun@163.com
  • 基金资助:

    成都市卫生局2009年重大科技攻关课题

Drug susceptibility testing and randomly amplified polymorphic DNA analysis of Staphylococcus aureus from 178 children with impetigo

  • Received:2012-01-06 Revised:2012-03-02 Online:2012-11-15 Published:2012-10-31

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摘要：

目的 探讨儿童脓疱疮皮损中金黄色葡萄球菌的耐药情况，比较敏感株与耐药株的DNA指纹差异。方法 对成都地区178例儿童脓疱疮患者皮损分泌物进行细菌培养，对培养出的162株金黄色葡萄球菌（简称金葡菌）进行21种抗生素的药物敏感试验，同时对162株金葡菌进行随机扩增多态性DNA分析。 结果 从脓疱疮患儿皮损中分离鉴定出病原菌180株，其中金葡菌162株，占90.00％。162株金葡菌中，148株为甲氧西林敏感金葡菌（MSSA），14株为耐甲氧西林金葡菌（MRSA）。162株金葡菌进行21种抗生素体外药敏试验，敏感率前5位分别为米诺环素、替考拉宁、喹奴普汀、万古霉素、呋喃妥因。耐药率前5位分别为青霉素、红霉素、克林霉素、复方磺胺甲■唑、四环素。未发现对夫西地酸、呋喃妥因、万古霉素、喹奴普汀、替考拉宁及米诺环素耐药。按DNA条带的大小和数量进行分型，共分为8种基因型，基因型Ⅲ最多占31.48%；基因型Ⅱ占26.54%；基因型Ⅵ占25.93%，这3种基因型占总数的65.43%（106/162）。148株MSSA的基因型有8种，基因型Ⅲ占33.78%，基因型Ⅵ占26.35%，基因型Ⅱ占22.30%。14株MRSA的基因型只有3种，分别为基因型Ⅱ10株（71.43%），基因型Ⅵ 3株（21.43%），基因型Ⅲ1株（7.14%），以基因型Ⅱ为主。结论 成都儿童脓疱疮皮损中病原菌以金葡菌为主，对米诺环素、替考拉宁及喹奴普汀等高度敏感。其RAPD指纹共分为8型，以基因型Ⅲ最多。

关键词: 随机扩增多态性DNA

Abstract:

Objective To test the antimicrobial susceptibility of Staphylococcus aureus from children with impetigo, and to assess the differences in randomly amplified polymorphic DNA profiles between sensitive and resistant Staphylococcus aureus strains. Methods Secretion specimens were obtained from the impetiginous lesions of 178 children, and subjected to bacterial culture. The susceptibility of 162 Staphylococcus aureus isolates against 21 antibiotics was tested. Randomly amplified polymorphic DNA PCR （RAPD-PCR） was performed to characterize the genotype of Staphylococcus aureus. Results Totally, 180 bacterial strains were isolated from 178 children with impetigo in Chengdu, including 162（90.00%） Staphylococcus aureus strains. Of the 162 Staphylococcus aureus strains, 148 were methicillin sensitive Staphylococcus aureus （MSSA）, 14 methicillin resistant Staphylococcus aureus （MRSA）. The most active antibiotic was minocycline, followed by teicoplanin, quinupristin, vancomycin and nitrofurantoin, while the resistance rate to penicillin was highest, followed by that to erythromycin, clindamycin, compound sulfamethoxazole and tetracycline. All the Staphylococcus aureus isolates were sensitive to fusidic acid, nitrofurantoin, vancomycin, minocycline and teicoplanin. According to RAPD-PCR, the 162 Staphylococcus aureus strains were divided into 8 genotypes, with the three most prevalent genotypes being Ⅲ（31.48%）, Ⅱ（26.54%） and Ⅵ（25.93%）, which accounted for 65.43% （106/162） in all the strains. The 148 MSSA strains fell into 8 genotypes, with genotype Ⅲ （50 strains, 33.78%）, Ⅵ （39 strains, 26.35%） and Ⅱ （33 strains, 22.30%） being the most prevalent genotypes; the 14 MRSA strains fell into 3 genotypes, i.e., genotypeⅡ （10 strains, 71.43%）,Ⅵ （3 strains, 21.43%）, and Ⅲ （1 strain, 7.14%）. Conclusions Staphylococcus aureus is the most prevalent pathogenic bacteria in children with impetigo in Chengdu area, which is highly sensitive to minocycline, teicoplanin and quinupristin, and falls into 8 genotypes according to RAPD-PCR with genotype Ⅲ being the most common genotype.

Key words: Randomly Amplified Polymorphic DNA

中图分类号: 

  • R753.1